Anti-RPE65 Polyclonal Antibody for WB, IF-P, ELISA - Q16518

Core Product Specifications and Parameters

Biological Background: RPE65 Function and Localization

• RPE65, also known as p63, BCO3, LCA2, and RP20, is a retinal pigment epithelium-specific 65 kDa protein essential for vision.
• It functions as a critical isomerohydrolase in the retinoid cycle, converting all-trans-retinyl esters to 11-cis-retinol, which is further oxidized to the chromophore 11-cis-retinal necessary for rod and cone photoreceptor function. Related references: PMID:16116091 PMID:17848510
• RPE65 also acts as a lutein isomerase, catalyzing the conversion of lutein to meso-zeaxanthin, an eye-specific carotenoid. Related references: PMID:28874556
• The soluble form binds all-trans-retinol (vitamin A), while the membrane‑associated, LRAT-palmitoylated form binds all‑trans‑retinyl esters, facilitating isomerohydrolase activity.
• Subcellularly, RPE65 localizes to the cytoplasm, cell membrane, and microsome membrane.
• Post‑translational modifications, including palmitoylation, phosphorylation, and acetylation, regulate its activity and localization.
• Mutations in the RPE65 gene cause Leber congenital amaurosis (LCA2) and retinitis pigmentosa, highlighting its critical role in retinal health.

Experimental Guidance and Technical Tips

• The immunogen (aa 274–533) targets a central region; consider verifying detection of full‑length and potential processed forms in your sample system.
• For Western blot, an expected band near 61 kDa should be observed; retinal pigment epithelium lysates may serve as a positive control.
• For immunofluorescence on paraffin sections, optimize antigen retrieval and validate signal in retinal tissue.
• Cross‑reactivity with mouse and rat RPE65 is reported; suitable for comparative studies across species.
• Due to multiple cellular localizations, subcellular fractionation can aid pathway-specific analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this RPE65 antibody, bulk ancillary reagents, and specialized sourcing for rare targets. Whether you are developing ophthalmic diagnostics or studying visual cycle mechanisms, our supply network supports your research with reliable, high-quality materials from concept to clinic.