Anti-RPE65 Polyclonal Antibody for WB and ELISA - Q16518

Core Product Specifications and Parameters

Biological Background: RPE65 Function and Localization

• RPE65, also known as retinoid isomerohydrolase, p63, BCO3, LCA2, RP20, rd12, mRPE65, and sRPE65, is a key enzyme in the visual cycle.
• It catalyzes the isomerohydrolase cleavage of all-trans-retinyl esters to 11-cis-retinol, a critical step in regenerating the visual chromophore 11-cis-retinal. Related references: PMID:16116091
• RPE65 is essential for the production of 11-cis retinal for both rod and cone photoreceptors, as demonstrated in functional studies. Related references: PMID:17848510
• The enzyme also exhibits isomerase activity towards lutein, converting it to meso-zeaxanthin, an eye-specific carotenoid. Related references: PMID:28874556
• Subcellularly, RPE65 localizes to the cytoplasm, cell membrane, and microsomal membranes, with its membrane association regulated by palmitoylation.
• Its expression is highly specific to the retinal pigment epithelium (RPE) at the protein level.
• Mutations in the RPE65 gene are linked to inherited retinal dystrophies, including Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa type 20 (RP20).
• Post-translational modifications such as acetylation, palmitoylation, and phosphorylation play important roles in modulating its activity and subcellular trafficking.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 340–440 of human RPE65, a region well conserved between human and mouse, which likely explains the observed cross-reactivity. Users should verify reactivity with other species if needed by sequence alignment.
• For Western blotting (WB), the predicted molecular weight is 61 kDa. Include appropriate positive controls (e.g., retinal pigment epithelium lysates) and optimize blocking conditions to minimize non-specific bands.
• For ELISA, this polyclonal antibody can be explored as a capture or detection reagent. Titration in the specific assay buffer is recommended to determine the optimal working concentration.
• Since RPE65 is a membrane-associated protein, consider using lysis buffers containing mild detergents (such as 0.1% Triton X-100 or NP-40) to improve protein extraction efficiency for downstream applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with convenient access to high-quality IVD raw materials, including validated antibodies for vision-related targets such as RPE65. Our portfolio encompasses optimized monoclonal and polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents to support assay development. For rare or custom needs, CamelBio leverages extensive supply networks to source hard-to-find raw materials, helping accelerate your journey from concept to clinic.