Anti-RPE Polyclonal Antibody for WB - Q96AT9

Core Product Specifications and Parameters

Biological Background: RPE Function and Localization

• RPE (Ribulose-phosphate 3-epimerase), also known as RPE2-1, is encoded by the RPE gene in humans.
• It catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate, a key step in the pentose phosphate pathway.
• The enzyme belongs to the ribulose-phosphate 3-epimerase family within the isomerase class.
• RPE is a metal-dependent protein, with experimental evidence indicating binding to cofactors such as zinc, cobalt, iron, and manganese.
• Post-translational modifications include acetylation, and alternative splicing results in multiple transcript variants.
• Three-dimensional structures of RPE have been deposited in the PDB, aiding in understanding its catalytic mechanism.
• The predicted molecular weight of the canonical isoform is approximately 25 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the full-length sequence (aa 1-228) of human RPE. This design may present native epitopes, potentially enhancing recognition of the endogenous protein.
• For Western blotting, a predominant band near 25 kDa is expected. Include appropriate positive controls (e.g., human, mouse, or rat cell lysates) and validate with molecular weight markers.
• Optimize primary antibody dilutions for each application (WB, IF/ICC, ELISA) and cell type, as reactivity may vary.
• Fixation and permeabilization conditions for IF/ICC should be carefully titrated; over-fixation may mask the epitope.
• This antibody cross-reacts with human, mouse, and rat species; verify performance in the relevant model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing one-stop access to a broad catalog of IVD raw materials, including validated antibody pairs, optimized monoclonals and polyclonals, and bulk ancillary reagents. The RPE polyclonal antibody supports research in carbohydrate metabolism and related pathways, ideal for metabolic disorder biomarker development. Our team can source rare-target antibodies and tailor supply solutions to accelerate your diagnostic projects from concept to clinic.