Anti-RPB3/POLR2C Rabbit Polyclonal Antibody for WB, IP, ELISA - P19387

Core Product Specifications and Parameters

Biological Background: RPB3 (POLR2C) Function and Localization

• RPB3, encoded by the POLR2C gene, is a subunit of DNA-directed RNA polymerase II (Pol II), also known as RPB33, RPB31, hRPB33, and hsRPB3.
• As a core component of Pol II, RPB3 participates in DNA-dependent transcription, synthesizing mRNA precursors and many non-coding RNAs from ribonucleoside triphosphates.
• RPB3 is essential for the assembly and stability of the Pol II complex, contributing to the enzymatic machinery responsible for RNA synthesis.
• The protein localizes predominantly to the nucleus, consistent with its role in transcription.
• RPB3 is a phosphoprotein, suggesting potential regulation by phosphorylation events.
• Structural studies have determined the 3D configuration of Pol II, including the RPB3 subunit, providing insights into the transcription mechanism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-275 of human RPB3 (NP_116558.1), covering the full-length protein; consider this for detecting both native and recombinant RPB3.
• For Western blot (WB), the predicted molecular weight is approximately 31 kDa; validate the band in the relevant sample system, as post-translational modifications or isoforms may cause shifts.
• Immunoprecipitation (IP) and ELISA applications are supported; optimize antibody concentration and assay conditions for each specific experimental setup.
• Because the antibody is polyclonal and cross-reacts with mouse RPB3, it may be useful for comparative studies across human and mouse samples; confirm cross-reactivity in your tissue or cell line of interest.
• For nuclear targets like RPB3, ensure proper sample preparation and lysis protocols to release nuclear proteins effectively.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to validated IVD raw materials, including this anti-RPB3 polyclonal antibody for transcription and RNA polymerase II research. Our portfolio covers optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and rare-target raw-material sourcing. Partner with CamelBio for reliable supply and technical support spanning concept to clinic.