Anti-Rpb1 CTD Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ChIP, ELISA - P24928

Core Product Specifications and Parameters

Biological Background: Rpb1 CTD Function and Localization

• POLR2A encodes Rpb1, the largest catalytic subunit of DNA-directed RNA polymerase II (Pol II), responsible for synthesizing mRNA precursors and non-coding RNAs. The enzyme uses DNA as a template and ribonucleoside triphosphates to polymerize RNA, proceeding through initiation, elongation, and termination stages. (PubMed:23748380, PubMed:27193682, PubMed:30190596, PubMed:28108474, PubMed:8381534, PubMed:9852112) Related references: PMID:23748380, PMID:27193682, PMID:30190596, PMID:28108474, PMID:8381534, PMID:9852112
• The CTD of Rpb1, containing 52 tandem heptapeptide repeats, acts as a scaffold for transcription regulators. Phosphorylation at Ser5 (Ser5P) facilitates promoter escape, while Ser2 phosphorylation (Ser2P) is required for pause release and elongation. Additionally, CTD lysine acetylation regulates immediate early gene induction, and methylation/dimethylation generally repress target gene expression. (PubMed:24207025, PubMed:26124092) Related references: PMID:24207025, PMID:26124092
• Rpb1 possesses a 3'→5' exonuclease activity that proofreads nascent RNA, enabling backtracking and cleavage of misincorporated nucleotides. (PubMed:8381534) Related references: PMID:8381534
• Beyond its DNA-dependent role, Rpb1 exhibits RNA-dependent RNA polymerase activity, extending non-coding RNAs using an internal template, and can replicate Hepatitis delta virus genome when associated with the viral small delta antigen.
• Rpb1 localizes primarily to the nucleus, where transcription occurs, but has also been detected in the cytoplasm and on chromosomes, consistent with its roles in transcription and potential extranuclear functions.
• Post-translational modifications on Rpb1 include phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, dynamically regulating its activity and interactions.
• Alternative splicing of POLR2A yields multiple isoforms, though functional differences remain to be fully elucidated.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1611-1710 within the CTD, suggesting the antibody detects total Rpb1 or a specific CTD epitope; verify phospho-dependent reactivity using phosphatase-treated samples.
• For Western blotting, the full-length Rpb1 protein is predicted at ~217 kDa; additional bands (e.g., ~64 kDa) may represent isoforms or degradation products; validate with appropriate controls.
• This antibody has been applied in ChIP assays; optimize crosslinking and chromatin fragmentation conditions for your specific target loci.
• For immunofluorescence, expect nuclear staining; co-stain with a cytoplasmic marker to confirm localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including this anti-Rpb1 CTD monoclonal antibody for transcription and gene regulation studies. Whether you need validated antibody pairs, bulk reagents, or specialized sourcing for rare targets, our team ensures reliable supply and technical support. Streamline your assay development from concept to clinic with CamelBio's one-stop procurement solutions.