Anti-RPA70/RPA1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P27694

Core Product Specifications and Parameters

Biological Background: RPA70/RPA1 Function and Localization

• Replication protein A 70 kDa DNA-binding subunit (RPA70) is the largest subunit of the heterotrimeric RPA complex, which binds and stabilizes single-stranded DNA intermediates formed during DNA replication and repair, preventing reannealing and coordinating protein recruitment (PubMed:17596542, PubMed:27723717, PubMed:27723720). Related references: PMID:17596542, PMID:27723717, PMID:27723720
• Essential for DNA replication and the cellular response to DNA damage, the RPA complex orchestrates DNA repair and checkpoint activation (PubMed:9430682). Related references: PMID:9430682
• RPA recruits and activates ATR kinase via ATRIP, positioning it as a master regulator of the DNA damage response (PubMed:24332808). Related references: PMID:24332808
• It facilitates recruitment of RAD51 and RAD52 to chromatin at DNA double-strand breaks, thereby promoting homologous recombination repair (PubMed:17765923). Related references: PMID:17765923
• RPA70 directly interacts with nucleotide excision repair factors XPA and XPG and is required for this repair pathway (PubMed:7697716). Related references: PMID:7697716
• Additionally, RPA70 participates in base excision repair through UNG interaction (PubMed:9765279), contributes to telomere maintenance (PubMed:17959650, PubMed:34767620), and as part of the alternative aRPA complex, may support DNA repair but not chromosomal DNA replication (PubMed:19996105). Related references: PMID:9765279, PMID:17959650, PMID:34767620, PMID:19996105
• Subcellularly, RPA70 localizes to the nucleus and PML bodies, consistent with its DNA metabolic functions.
• Extensive post-translational modifications, including phosphorylation, acetylation, ADP-ribosylation, and ubiquitination, regulate its activity; the protein contains zinc-finger domains essential for DNA binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal 1–240 amino acids of human RPA70. For Western blot, expect a specific band at approximately 68 kDa in human cell lysates; consider using nuclear extracts to enrich the signal.
• For immunohistochemistry (IHC‑P), antigen retrieval may be required; nuclear staining is anticipated. Validate staining patterns in appropriate positive and negative control tissues.
• This polyclonal antibody is suitable for ELISA; optimization of coating concentration and blocking conditions is recommended.
• Because RPA70 is highly conserved, cross‑reactivity with other species (e.g., mouse, rat) might be possible but is not guaranteed; verify in the intended species if used beyond human.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, supporting diagnostic manufacturers from concept to clinic. This RPA70/RPA1 antibody is ideal for DNA replication and repair research, and CamelBio can supply it along with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare‑target sourcing. Our one‑stop platform streamlines procurement, ensuring you have the critical tools to advance your diagnostic development efficiently.