Anti-RPA32/RPA2 Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P15927

Core Product Specifications and Parameters

Biological Background: RPA32/RPA2 Function and Localization

• RPA2 encodes the 32 kDa subunit of the heterotrimeric replication protein A (RPA) complex, also known as replication factor A protein 2 (RF-A p2) or RP-A p34.
• The RPA complex binds and stabilizes single-stranded DNA (ssDNA) intermediates formed during DNA replication or upon DNA stress, preventing reannealing and recruiting proteins involved in DNA metabolism.
• In the DNA damage response, RPA2 facilitates repair and checkpoint activation by recruiting ATRIP/ATR kinase; recruits RAD51 and RAD52 for double-strand break repair; recruits XPA and XPG for nucleotide excision repair; and interacts with UNG for base excision repair.
• RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ. Related references: PMID:17596542
• The RPA complex also contributes to telomere maintenance.
• Subcellular localization includes nucleus and PML bodies.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination regulate RPA2 function; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-270, covering nearly the full-length protein; the antibody is likely to recognize native conformations suitable for multiple applications.
• In Western blotting, expect a band approximately 29 kDa; validate in human, mouse, and rat lysates.
• For immunofluorescence and immunohistochemistry, anticipate nuclear staining consistent with RPA2 localization; consider appropriate fixation and permeabilization protocols.
• For ELISA, titrate the antibody to optimize signal-to-noise ratio in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated monoclonal and polyclonal antibodies, antibody pairs, and ancillary reagents. This anti-RPA2 antibody supports investigations into DNA replication and DNA damage response pathways. For specialized targets like RPA2, CamelBio streamlines procurement from diverse sources, accelerating your assay development from concept to clinic.