Anti-RORβ Rabbit Monoclonal Antibody for WB, ELISA - Q92753

Core Product Specifications and Parameters

Biological Background: RORβ Function and Localization

• RORβ (NR1F2, RZRB) is a nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a core motif half-site 5'-AGGTCA-3'. It exhibits intrinsic transcriptional activity and can be repressed by inverse agonists such as all-trans retinoic acid.
• Essential for normal postnatal development of rod and cone photoreceptor cells; modulates rod differentiation via NRL-mediated pathway and regulates cone opsin OPN1SW expression.
• Isoform 1 is critical for hindlimb motor control and retinal interneuron differentiation, synergistically regulating PTF1A with FOXN4.
• Involved in circadian rhythm regulation, controlling period length and stability.
• May contribute to neocortical cytoarchitectural patterning during development and dose-dependent barrel formation in layer IV neurons.
• Localized to the nucleoplasm, consistent with its role as a transcription factor.
• Keywords: Activator, Alternative promoter usage, Biological rhythms, Developmental protein, DNA-binding, Metal-binding, Nucleus, Receptor, Sensory transduction, Transcription regulation, Vision, Zinc-finger.

Experimental Guidance and Technical Tips

• The immunogen covers N-terminal amino acids 1–100; verify detection specificity for full-length and potential N-terminal isoforms in your experimental context.
• For Western blot, optimize using lysates from tissues with known RORβ expression (e.g., retina, brain) from human, mouse, or rat.
• In ELISA applications, consider direct or competitive formats using the immunogen peptide or recombinant protein as coated antigen.
• Cross-reactivity with mouse and rat indicates potential for use in rodent models; validate with appropriate controls and species-specific conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with comprehensive IVD raw-material sourcing, technical services, and consulting. This anti-RORβ rabbit monoclonal antibody exemplifies our capability to deliver high-quality research reagents for targets involved in circadian biology and neurodevelopment. Beyond single antibodies, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, supporting you from concept to clinic with a single integrated partner.