Anti-RORC Polyclonal Antibody for WB, ELISA - P51449

Core Product Specifications and Parameters

Biological Background: RORC Function and Localization

• RORC encodes the nuclear receptor ROR-gamma (NR1F3), a member of the ROR subfamily of nuclear hormone receptors. Two isoforms exist: isoform 1 is widely expressed, while isoform 2 is predominantly found in immature thymocytes.
• RORC regulates cellular differentiation, immunity, metabolism (glucose, lipid, steroid, xenobiotics), and peripheral circadian rhythms. It binds to ROR response elements (RORE) and its activity can be modulated by ligands like oxysterols. Related references: PMID:19381306, PMID:19965867, PMID:20203100, PMID:22789990
• It is essential for thymopoiesis, lymph node and Peyer's patch development, Th17 cell differentiation, and interferon-gamma-dependent anti-mycobacterial immunity. Related references: PMID:21499262, PMID:26160376
• RORC is a nuclear protein that regulates target gene transcription by recruiting coactivators or corepressors.
• It contains zinc finger motifs typical of nuclear receptors, facilitating DNA binding and transcriptional regulation.
• Isoform 1 is highly expressed in skeletal muscle, liver, and adipose tissue; isoform 2 is primarily thymus-specific.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 223-312) corresponds to the ligand-binding domain of human RORC, a region well-conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, a primary band at approximately 58 kDa is expected. Due to potential alternative splicing or post-translational modifications, additional bands may appear; titrate antibody concentration and validate in your specific sample system.
• This polyclonal antibody is suitable for ELISA. Consider optimizing coating conditions and using it as a capture or detection reagent in sandwich ELISA formats.
• Ensure appropriate blocking and wash steps to minimize background, as polyclonal antibodies can generate higher non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a reliable one-stop supplier of IVD raw materials, providing antibodies like this anti-RORC polyclonal antibody for research and diagnostic development. With a focus on immune-oncology and metabolic disease targets, CamelBio supports your projects with a wide range of validated reagents and custom sourcing services, helping bridge the gap from concept to clinical application.