Anti-RORA Polyclonal Antibody for WB, ELISA - P35398

Core Product Specifications and Parameters

Biological Background: RORA Function and Localization

• RORA encodes the nuclear receptor ROR-alpha, a ligand-dependent transcription factor that binds as a monomer to ROR response elements (RORE) to regulate gene expression.
• Acts as a key regulator of embryonic development, cellular differentiation, immunity, and circadian rhythm; its transcriptional activity can be modulated by oxysterols such as 25-hydroxycholesterol (agonist) or 7-oxygenated sterols (inverse agonist).
• Required for proper cerebellum development, where it regulates granule cell proliferation via SHH gene expression and calcium-mediated signal transduction. Related references: PMID:29656859
• Competes with the repressor NR1D1 for binding to RORE sites in clock gene promoters (e.g., BMAL1, CRY1), thereby driving rhythmic expression and influencing circadian period length and stability.
• Modulates hepatic lipid and glucose metabolism by regulating genes such as APOA1, APOA5, APOC3, PPARG, CYP7B1, SULT2A1, G6PC1, and PCK1; also cooperates with NR1H2/NR1H3 in cholesterol metabolism.
• Promotes Th17 cell lineage commitment downstream of IL‑6 and TGF‑β, through direct regulation of IL17 and IL17F expression; antagonizes Th1 differentiation.
• Widely expressed in various tissues, including regulatory and effector T-cells (PubMed:18354202, PubMed:7916608). Isoform 4 is highly expressed in the central nervous system and cerebellum (PubMed:29656859). Related references: PMID:18354202 PMID:7916608 PMID:29656859
• Contains a zinc-finger DNA-binding domain and is subject to post-translational modifications including phosphorylation, methylation, isopeptide bond formation, and ubiquitin-like conjugation.
• Multiple protein isoforms are produced by alternative promoter usage and splicing, with apparent molecular weights ranging from 53 to 63 kDa.

Experimental Guidance and Technical Tips

• This polyclonal antibody is suitable for Western blot (WB) and ELISA applications. For WB, consider starting with a standard dilution range and optimizing based on your sample type and detection system.
• Due to alternative splicing, RORA may appear as multiple bands between 53 and 63 kDa. Confirm the expected isoform pattern in your target tissue or cell line.
• The antibody cross-reacts with human, mouse, and rat RORA. Validate reactivity in your specific species and sample matrix before large-scale experiments.
• For ELISA, titrate the antibody and use appropriate coating antigens (e.g., recombinant RORA fragments) to establish optimal signal-to-noise ratios.
• Include appropriate controls (e.g., lysates from tissues with known RORA expression, siRNA knockdown samples) to verify specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a single-source partner for IVD raw materials, offering a wide range of validated antibodies and reagents to accelerate your research and development. This RORA polyclonal antibody supports investigations into circadian biology, metabolic disorders, and immune cell differentiation. In addition to primary antibodies, we supply optimized antibody pairs, ancillary reagents, and can assist with sourcing rare target materials—streamlining your path from concept to clinic.