Anti-ROR2 Rabbit Polyclonal Antibody for WB, ELISA - Q01974

Core Product Specifications and Parameters

Biological Background: Tyrosine-protein kinase transmembrane receptor ROR2 Function and Localization

• ROR2 is a tyrosine-protein kinase receptor also known as neurotrophic tyrosine kinase receptor-related 2 (NTRKR2).
• It is involved in early chondrocyte formation and is required for cartilage and growth plate development (by similarity).
• Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation. Related references: PMID:17717073
• In vitro studies have shown that ROR2 may exhibit very little tyrosine kinase activity.
• Functions as a receptor for WNT5A, potentially inhibiting WNT3A-mediated signaling. Related references: PMID:25029443
• Localized to the cell membrane as a single-pass type I membrane protein.
• Features an immunoglobulin domain, kringle domain, and kinase domain; undergoes glycosylation, phosphorylation, and other post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100–200 within the extracellular domain of human ROR2; consider validating antibody specificity using knockout or overexpression models.
• For Western blotting, the predicted molecular weight is ~105 kDa; gel percentage and transfer conditions should be optimized accordingly.
• The antibody cross-reacts with mouse and rat ROR2, enabling studies in rodent models of skeletal development and Wnt signaling.
• In ELISA applications, titrate antibody concentrations and validate with appropriate positive and negative controls to ensure robust signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive sourcing platform for IVD raw materials. This anti-ROR2 polyclonal antibody supports investigations into Wnt signaling, bone formation, and skeletal development. Beyond this target, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets—streamlining your journey from concept to clinic.