Anti-ROR1 Polyclonal Antibody for WB, ELISA - Q01973

Core Product Specifications and Parameters

Biological Background: ROR1 Function and Localization

• ROR1 (Neurotrophic tyrosine kinase, receptor-related 1) is an inactive tyrosine-protein kinase transmembrane receptor, encoded by the ROR1 (NTRKR1) gene, with a molecular weight of approximately 104 kDa.
• It acts as a receptor for WNT5A, activating downstream NF-κB signaling and potentially inhibiting WNT3A-mediated signaling (PubMed:25029443, PubMed:27162350). Related references: PMID:25029443 PMID:27162350
• Through IGFBP5 ligand, ROR1 forms a complex with ERBB2 to enhance CREB oncogenic signaling (PubMed:36949068). Related references: PMID:36949068
• In the inner ear, ROR1 is crucial for spiral ganglion neurons to innervate auditory hair cells, and mutations are associated with non-syndromic deafness (PubMed:27162350). Related references: PMID:27162350
• Subcellularly, ROR1 localizes to the cell membrane and specifically to axon projections.
• Tissue expression: ROR1 is strongly expressed in heart, lung, and kidney, but weakly in the CNS; isoform Short is highly expressed in fetal and adult CNS and a variety of human cancers.
• The protein features immunoglobulin-like, kringle, and transmembrane domains, and is subject to alternative splicing and glycosylation.
• Keywords from UniProt include: Alternative splicing, Cell membrane, Cell projection, Deafness, Disease variant, Glycoprotein, Wnt signaling pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 24–403) of human ROR1, encompassing immunoglobulin and kringle domains, which may confer reactivity against both full-length and short isoforms.
• For Western blot, consider using reducing conditions and including positive control lysates from ROR1‑expressing cancer cell lines or tissues (e.g., heart, lung).
• When developing an ELISA, pairing this antibody with a detection antibody that targets a non‑overlapping epitope could be explored.
• Given the cross‑reactivity with rat, rat‑derived samples may serve as an alternative model system for validation.
• Since ROR1 exhibits very low intrinsic kinase activity, functional studies may focus on protein–protein interactions or downstream signaling events (e.g., NF‑κB activation).

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