Anti-ROM1 Polyclonal Antibody for WB, IHC-P, ELISA - Q03395

Core Product Specifications and Parameters

Biological Background: ROM1 Function and Localization

• Rod outer segment membrane protein 1 (ROM1), also known as tetraspanin-23 (Tspan-23), is a transmembrane protein belonging to the tetraspanin family.
• ROM1 plays a critical role in rod outer segment (ROS) morphogenesis and the maintenance of the structural integrity of ROS curved disks, likely in cooperation with peripherin-2 (PRPH2).
• It is involved in the organization of the ROS and maintenance of ROS disk diameter, as well as the preservation of the retina outer nuclear layer.
• The protein localizes to the photoreceptor inner segment membrane and outer segment membrane, specifically enriched in the rim region of ROS disks.
• At the protein level, ROM1 is detected in retina photoreceptors, as shown by immunolocalization studies that highlight its presence in the disk rims. Related references: PMID:1610568, PMID:8504299
• It contains conserved tetraspanin features, including four transmembrane domains and disulfide bonds, and is implicated in cell adhesion and sensory transduction.
• Mutations in ROM1 are associated with retinitis pigmentosa, a degenerative retinal disease affecting vision.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment corresponding to amino acids 126–263 of human ROM1, which may aid in specific detection across species where this region is conserved.
• The antibody has been applied in Western blot (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA. Consider validating these applications in your model system, especially using retinal tissue or cell lysates.
• For IHC-P, antigen retrieval conditions may need optimization, and endogenous peroxidase blocking is recommended for retinal tissues.
• The predicted molecular weight of ROM1 is approximately 37 kDa, but post-translational modifications or dimerization may result in bands at different apparent sizes in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-ROM1 reagent. Beyond this product, we offer bulk ancillary reagents and can assist in sourcing rare target raw materials for retinal and vision research applications. From concept to clinic, CamelBio supports your development pipeline with reliable procurement and technical guidance.