Anti-ROCK1 Rabbit Polyclonal Antibody (KO Validated) for WB, IHC-P, ELISA - Q13464

Core Product Specifications and Parameters

Biological Background: ROCK1 Function and Localization

• ROCK1 encodes Rho-associated protein kinase 1, a serine/threonine kinase of approximately 158 kDa, featuring coiled-coil, zinc-finger, and pleckstrin homology domains.
• It is a key regulator of the actin cytoskeleton and cell polarity, mediating smooth muscle contraction, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of substrates such as LIMK1, LIMK2, MYL9/MLC2, and PPP1R12A.
  Related references: PMID:10436159, PMID:10652353, PMID:11018042
• ROCK1 phosphorylates FHOD1 to promote SRC-dependent non-apoptotic plasma membrane blebbing.
  Related references: PMID:18694941
• It phosphorylates JIP3, regulating JNK recruitment upon UVB-induced stress.
  Related references: PMID:19036714
• ROCK1 suppresses inflammatory cell migration by regulating PTEN stability, and negatively regulates VEGF-induced angiogenic endothelial cell activation.
  Related references: PMID:19181962
• Subcellularly, ROCK1 localizes to the cytoplasm, cytoskeleton, centrosome, Golgi apparatus, cell membrane, and cell projections like blebs, lamellipodia, and ruffles.
• It is involved in centrosome positioning, terminal erythroid differentiation, and keratinocyte terminal differentiation.
  Related references: PMID:21072057, PMID:19997641
• ROCK1 participates in osteoblast compaction and closure of eyelids and ventral body wall, and is detected in blood platelets.

Experimental Guidance and Technical Tips

• As a polyclonal antibody, it recognizes multiple epitopes within the immunogen region (aa 400–660), potentially offering robust signal detection.
• For Western blot, a band near 158 kDa is expected; validate in your specific sample system using appropriate loading controls and consider knockout validation.
• In immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization; include tissue controls with known ROCK1 expression.
• For ELISA, the recombinant immunogen can serve as a positive control; ensure antibody dilution is optimized for your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for high-quality IVD raw materials, from concept to clinic. For studies on cytoskeleton dynamics and cell signaling, we offer this ROCK1 polyclonal antibody alongside a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. Our sourcing services ensure timely access to rare and specialized targets to accelerate your diagnostic development.