Anti-ROCK1 Rabbit Polyclonal Antibody for WB, IP, IF, ELISA - Q13464

Core Product Specifications and Parameters

Biological Background: ROCK1 Function and Localization

• ROCK1 is a serine/threonine-protein kinase and member of the Rho-associated coiled-coil-containing protein kinase family. It is also known as ROCK-I or p160ROCK, and was identified as a renal carcinoma antigen NY-REN-35.
• ROCK1 is a key regulator of the actin cytoskeleton and cell polarity, controlling smooth muscle contraction, actin organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility through phosphorylation of substrates like DAPK3, GFAP, LIMK1/2, MYL9/MLC2, PFN1 and PPP1R12A. Related references: PMID:10436159, PMID:10652353, PMID:11018042
• ROCK1 phosphorylates FHOD1 and synergistically promotes SRC-dependent non-apoptotic plasma membrane blebbing. Related references: PMID:18694941
• Upon UVB-induced stress, ROCK1 phosphorylates JIP3 and regulates the recruitment of JNK to JIP3. Related references: PMID:19036714
• Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Related references: PMID:19181962
• Promotes keratinocyte terminal differentiation. Related references: PMID:19997641
• Plays a role in terminal erythroid differentiation. Related references: PMID:21072057
• ROCK1 localizes to the cytoplasm, cytoskeleton, centrosome, centriole, Golgi apparatus membrane, and cell membrane. It is also found at cell projections including blebs, lamellipodia, and ruffles.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 400–660 within the N-terminal half of ROCK1, which may allow recognition of full-length protein and potentially some isoforms. Verify reactivity in the relevant sample system.
• For Western blot (WB), the predicted molecular weight is approximately 158 kDa. Use appropriate gel percentage (e.g., 6–8% SDS‑PAGE) and optimize transfer conditions for high‑molecular‑weight proteins.
• For immunoprecipitation (IP), titrate the antibody amount and select a suitable binding/wash buffer to maximize capture efficiency.
• For immunofluorescence on paraffin‑embedded sections (IF‑P), consider heat‑induced antigen retrieval and include both positive and negative control tissues.
• For ELISA, test against recombinant ROCK1 protein or native lysates to determine optimal coating and detection conditions. Validate with known positive/negative samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. With our anti‑ROCK1 polyclonal antibody, researchers investigating actin cytoskeleton dynamics, cell motility, and cancer can source validated reagents for immunoassays. Beyond antibodies, CamelBio offers optimized antibody pairs, bulk ancillary reagents, and rare‑target raw‑material sourcing to streamline your diagnostic development pipeline.