Anti-ROBO1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y6N7

Core Product Specifications and Parameters

Biological Background: ROBO1 Function and Localization

• ROBO1 (Roundabout homolog 1), also known as DUTT1 (Deleted in U Twenty Twenty) or H-Robo-1, is a receptor for SLIT1 and SLIT2 guidance cues.
• It mediates axonal navigation at the ventral midline of the neural tube and projection of axons during neuronal development. Related references: PMID:10102268, PMID:24560577
• Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. Related references: PMID:24560577
• The protein is localized at the cell membrane, axon projections, and the endoplasmic reticulum-Golgi intermediate compartment membrane.
• It is widely expressed across tissues, with the exception of kidney.
• ROBO1 regulates cell migration by interacting with MYO9B, leading to increased levels of active, GTP-bound RHOA. Related references: PMID:26529257
• The protein contains immunoglobulin domains, fibronectin type III repeats, and a transmembrane domain, and is subject to glycosylation, phosphorylation, and ubiquitination.
• Alternatively spliced isoforms and disease-associated variants (e.g., CPHD8, dwarfism, intellectual disability) highlight its importance in development and disease.

Experimental Guidance and Technical Tips

• For Western blot, anticipate a band around 181 kDa; include molecular weight markers and validate with a known positive control (e.g., HEK293 or mouse brain lysate).
• The immunogen spans amino acids 1452–1651 in the C-terminal region; confirm specificity by pre-incubating the antibody with the immunogen peptide in blocking experiments.
• For IF/ICC, use appropriate cell lines expressing ROBO1 (e.g., neuroblastoma or cortical neuron cultures) and perform permeabilization steps; co-staining with axonal markers can help verify localization.
• Given the cross-reactivity with mouse and rat, this antibody may be suitable for rodent models of neurodevelopment; perform initial titration in the intended species.
• In ELISA, consider using recombinant ROBO1 extracellular domain fragments as capture reagents and validate with SLIT1/2 binding competition assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials. This anti-ROBO1 polyclonal antibody exemplifies our commitment to supporting axon guidance and neurodevelopment research, offering validated reagents for WB, IF/ICC, and ELISA. Beyond antibodies, we provide matched antigen pairs, optimized monoclonal and polyclonal panels, bulk ancillary reagents, and custom sourcing for rare targets—ensuring seamless integration from assay development to clinical translation.