Anti-RNF8 Polyclonal Antibody for WB, ELISA - O76064

Core Product Specifications and Parameters

Biological Background: RNF8 Function and Localization

• RNF8 is an E3 ubiquitin-protein ligase that plays a central role in DNA damage signaling by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX at double-strand breaks (DSBs), thereby promoting the recruitment of DNA repair proteins such as TP53BP1 and BRCA1 (PubMed:18001824, 18006705). Related references: PMID:18001824, PMID:18006705
• It also catalyzes 'Lys-48'-linked polyubiquitin chains, targeting substrates like JMJD2A/KDM4A for degradation, which unmasks H4K20me2 marks and facilitates TP53BP1 recruitment at DNA damage sites (PubMed:11322894, 14981089, 17724460). Related references: PMID:11322894, PMID:14981089, PMID:17724460
• RNF8 contributes to interstrand cross-link repair and Fanconi anemia pathway activation through H2A/H2AX ubiquitination, and promotes non-homologous end joining by degrading KU80/XRCC5.
• It is involved in higher-order chromatin decompaction and regulates histone modifications, including H2B ubiquitination and H4K16 acetylation, to facilitate ATM kinase activation.
• Subcellular localization includes the nucleus, cytoplasm, midbody, and telomeres, consistent with its roles in DNA repair, mitosis, and telomere maintenance.
• Ubiquitously expressed, with highest levels in adult brain and testis, and plays a critical role in spermatogenesis by mediating histone replacement.
• Additional functions include immune class switch recombination (PubMed:22865450) and potential regulation of RXRA transcriptional activity. Related references: PMID:22865450

Experimental Guidance and Technical Tips

• For western blotting, whole-cell lysates from human, mouse, or rat cell lines are appropriate; the predicted molecular weight is approximately 56 kDa, but post-translational modifications may result in additional bands.
• The antibody is suitable for ELISA; consider using the recombinant immunogen (amino acids 230-485) as a positive control.
• As a polyclonal antibody, lot-to-lot consistency should be verified; validate performance in the specific experimental context.
• To enhance detection of DNA damage-related RNF8 activity, consider inducing DSBs with ionizing radiation or chemical agents prior to sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, connecting researchers and diagnostic manufacturers with validated antibodies, optimized reagents, and bulk ancillary materials. For studies on DNA damage repair and ubiquitin signaling pathways, this anti-RNF8 polyclonal antibody is part of a broader portfolio addressing critical research areas. Our sourcing capabilities also include rare-target antibody development and tailored solutions to accelerate your assay and diagnostic development workflows.