Anti-RNF40 Rabbit Polyclonal Antibody for WB and ELISA - O75150

Core Product Specifications and Parameters

Biological Background: RNF40 Function and Localization

• RNF40 (also known as BRE1B, RBP95, STARING) is the E3 ubiquitin-protein ligase BRE1B, a component of the RNF20/40 complex that catalyzes monoubiquitination of histone H2B at Lys-120 (H2BK120ub1).
• H2BK120ub1 is a key epigenetic mark for transcriptional activation and is required for subsequent methylation of histone H3 at Lys-4 and Lys-79 (H3K4me and H3K79me), linking it to gene regulation.
• The RNF20/40 complex functions in cooperation with the E2 enzymes UBE2A or UBE2B, and is essential for the activation of Hox genes.
• RNF40 localizes to the nucleus, consistent with its role in chromatin modification.
• Ubiquitously expressed, with higher levels in testis, heart, and pancreas, and lower in lung, skeletal muscle, and small intestine.
• Contains a RING-type zinc finger domain and participates in host-virus interactions, promoting the lytic cycle of human herpesvirus 8 (KSHV) by inducing viral gene expression.
• Post-translational modifications include acetylation, phosphorylation, and isopeptide bond formation (ubiquitination-related).

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-300), which includes the RING finger domain; the antibody may recognize full-length RNF40 and isoforms containing this region.
• For Western blot, a band at ~114 kDa is expected consistent with the calculated molecular weight; consider using nuclear extracts for enhanced detection due to nuclear localization.
• This antibody has been validated with human samples; cross-reactivity with other species may require additional experimental verification.
• ELISA performance may be optimized by using the immunogen peptide or recombinant protein as coating antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials, technical services, and expert consulting. The RNF40 Rabbit Polyclonal Antibody supports epigenetic and chromatin biology research, and CamelBio can supply validated antibody pairs, bulk ancillary reagents, and assist with custom sourcing for rare or difficult targets.