Anti-RNF34 Rabbit Polyclonal Antibody for WB, IF/ICC, and ELISA - Q969K3

Core Product Specifications and Parameters

Biological Background: RNF34 Function and Localization

• RNF34 is an E3 ubiquitin-protein ligase that catalyzes the ubiquitin-mediated proteasomal degradation of target proteins, including CASP8 and CASP10, thereby negatively regulating extrinsic apoptosis. Related references: PMID:15069192
• It directly ubiquitinates p53/TP53, targeting it for proteasomal degradation and thus negatively regulating p53-dependent cellular functions. Related references: PMID:17121812
• RNF34 indirectly regulates p53/TP53 by mediating the ubiquitination and degradation of SFN (14-3-3 sigma), a p53 stabilizer. Related references: PMID:18382127
• It mediates PPARGC1A (PGC-1α) proteasomal degradation, influencing brown fat cell metabolism and energy homeostasis. Related references: PMID:22064484
• Through Lys-48-linked polyubiquitination of NOD1, RNF34 promotes its proteasomal degradation, suggesting a role in regulating innate immunity. Related references: PMID:25012219
• The protein is localized to the cell membrane, endomembrane system, nucleus (including nuclear speckles), and cytosol.
• RNF34 is ubiquitously expressed across tissues such as heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon, and leukocytes.
• Structural features include a RING-type zinc finger; the protein is subject to alternative splicing and phosphorylation, consistent with its role in apoptosis and ubiquitination pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-220 amino acids of human RNF34. This region may be sufficient to detect full-length RNF34; however, endogenous RNF34 might exhibit multiple isoforms due to alternative splicing. Consider validating the antibody’s specificity in your target cell lines using appropriate controls.
• For Western blot (WB), the predicted molecular weight of RNF34 is approximately 42 kDa. A band near this size can be expected, but post-translational modifications or isoform variability may lead to slight shifts. Optimize blocking and antibody dilution for your samples.
• In immunofluorescence (IF/ICC), RNF34 has been reported to localize to the nucleus (including nuclear speckles), cytosol, and cell membrane. Proper fixation and permeabilization conditions should be tested to preserve antigenicity and achieve specific staining.
• For ELISA, the polyclonal nature of the antibody may provide high sensitivity for detecting RNF34 in human samples. Include standard curves and negative controls to ensure assay linearity and specificity.
• This antibody has been validated for human cross-reactivity. If using it for other species, sequence homology analysis and empirical testing are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, bridging diagnostic manufacturers, labs, and researchers from concept to clinic. This anti-RNF34 polyclonal antibody supports apoptosis and protein ubiquitination research, and CamelBio ensures reliable supply of optimized antibodies, ancillary reagents, and bulk materials. For specialized targets like rare E3 ligases, we offer sourcing of validated antibody pairs and recombinant proteins to streamline your assay development.