Anti-RNF31 Rabbit Polyclonal Antibody for WB, ELISA - Q96EP0

Core Product Specifications and Parameters

Biological Background: RNF31 Function and Localization

• RNF31 (HOIP) is the catalytic subunit of the linear ubiquitin chain assembly complex (LUBAC), essential for generating Met1-linked (linear) polyubiquitin chains and regulating NF-κB activation and inflammation. Related references: PMID:17006537 PMID:19136968 PMID:20005846
• LUBAC conjugates linear polyubiquitin to IKBKG (NEMO) and RIPK1, thereby activating the canonical NF-κB and JNK signaling pathways. Related references: PMID:17006537 PMID:19136968 PMID:20005846
• Linear ubiquitination by RNF31 interferes with TNF-induced cell death, preventing excessive inflammation. Related references: PMID:21455173 PMID:28189684
• RNF31 is recruited to the TNF-R1 signaling complex and contributes to complex stability by adding linear ubiquitin chains. Related references: PMID:20005846 PMID:27458237
• In innate immunity, RNF31 decorates intracellular bacteria with linear ubiquitin, marking them for xenophagy and triggering NF-κB responses. Related references: PMID:28481331 PMID:34012115
• Together with the deubiquitinase OTULIN, RNF31 regulates canonical Wnt signaling during angiogenesis. Related references: PMID:23708998
• RNF31 binds polyubiquitin chains of various linkage types, underscoring its broader role in ubiquitin signaling. Related references: PMID:23708998
• RNF31 localizes to the cytoplasm and is expressed in normal and transformed breast epithelial cell lines; it is a phosphoprotein with zinc-finger domains.

Experimental Guidance and Technical Tips

• For Western blot, the antibody detects a band at ~120 kDa; consider including appropriate positive and negative controls, especially when using human or rat samples.
• In ELISA, the immunogen region (aa 500–700) may capture both linear and conformational epitopes; optimize antigen coating and antibody concentrations for your specific assay format.
• Given RNF31’s role in signaling, validate the antibody in relevant biological contexts, such as TNF-α-stimulated cells, to confirm reactivity and functional performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials and technical support, from concept to clinic. This rabbit polyclonal antibody against RNF31 is just one example of our offerings for ubiquitination and NF-κB pathway studies. We supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, ensuring seamless procurement for your assay development.