Anti-RNF31 Polyclonal Antibody for WB, IF, ELISA - Q96EP0

Core Product Specifications and Parameters

Biological Background: RNF31 Function and Localization

• RNF31 (HOIP) is the catalytic component of the linear ubiquitin chain assembly complex (LUBAC), which generates Met1-linked (linear) polyubiquitin chains.
• LUBAC conjugates linear polyubiquitin to substrates like IKBKG (NEMO) and RIPK1, essential for canonical NF-κB and JNK signaling pathway activation. Related references: PMID:17006537, PMID:19136968, PMID:20005846
• Linear ubiquitination by LUBAC prevents TNF-induced cell death and inflammation, highlighting its anti-apoptotic and anti-inflammatory roles. Related references: PMID:21455173, PMID:28189684
• RNF31 is recruited to the TNF receptor signaling complex via polyubiquitin scaffolds formed by BIRC2/3, where it linearly ubiquitinates components to stabilize the complex. Related references: PMID:20005846, PMID:27458237
• In innate immunity, RNF31 and LUBAC conjugate linear polyubiquitin chains onto cytosolic bacteria, forming an 'eat-me' signal for xenophagy and promoting NF-κB activation. Related references: PMID:28481331, PMID:34012115
• RNF31 also linear ubiquitinates BCL10 to enhance TCR-induced NF-κB signaling and participates in Wnt pathway regulation during angiogenesis. Related references: PMID:27777308, PMID:23708998
• Predominantly localized to the cytoplasm, consistent with its role in cytosolic signaling complexes and bacterial coat formation.
• Expressed in both normal and transformed breast epithelial cell lines, with post-translational modifications including phosphorylation and zinc-binding domains.

Experimental Guidance and Technical Tips

• The immunogen spans residues 500-700, a region predicted to be surface-accessible and specific to RNF31, reducing cross-reactivity with other RING finger proteins.
• For Western blotting, expect a band near 120 kDa; optimize loading and transfer conditions for large proteins.
• In immunofluorescence (IF-P), nuclear and cytoplasmic staining may be observed depending on cell type and activation state; include appropriate controls.
• For ELISA, consider using the antibody as capture or detection reagent after confirming reactivity with native and recombinant RNF31.
• Cross-reactivity with mouse RNF31 is reported, but validate in your experimental system due to potential species-specific differences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For studies on ubiquitin signaling and NF-κB pathways, we offer this RNF31 polyclonal antibody as a reliable detection tool. If your project requires customized antibody formats, rare target material sourcing, or scale-up support, our technical team is ready to assist from concept to clinic.