Anti-RNF220 Rabbit Polyclonal Antibody for WB, ELISA - Q5VTB9

Core Product Specifications and Parameters

Biological Background: RNF220 Function and Localization

• RNF220 is a RING-type E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins, playing roles in Wnt signaling, neurodevelopment, and immune responses.
• It ubiquitinates and promotes proteasomal degradation of AMPA receptor subunits GRIA1 and GRIA2, thereby modulating synaptic activity and brain function. Related references: PMID:25266658, PMID:33964137
• Together with ZC4H2, RNF220 monoubiquitinates PHOX2A and PHOX2B, enhancing their DNA-binding ability and transactivity (By similarity).
• RNF220 stabilizes CTNNB1 (β-catenin) through USP7-mediated deubiquitination, thereby promoting Wnt signaling. Related references: PMID:25266658, PMID:33964137
• It regulates type I and type II interferon responses by mediating 'Lys-63'-linked polyubiquitination of STAT1, and also regulates STAT3 phosphorylation and transcriptional activity via ubiquitination. Related references: PMID:32814877, PMID:41219491
• RNF220 promotes 'Lys-63'-linked polyubiquitination and stabilization of OLIG1 and OLIG2, transcription factors essential for oligodendroglial development. Related references: PMID:38324685
• Subcellular localization: Found in both the cytoplasm and nucleus, consistent with its roles in nuclear signaling and cytoplasmic protein degradation.
• Tissue specificity: Ubiquitously expressed, with abundance in brain and spinal cord, particularly cerebellum and cerebral cortex; also highly expressed in fetal cerebellum, spinal cord, and cortex.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at ~63kDa; validate in human, mouse, or rat lysates. Consider initial dilutions of 1:500-1:2000 with appropriate controls.
• For ELISA, the polyclonal nature allows use as capture or detection antibody; optimize coating concentrations and assay conditions.
• Because the immunogen spans amino acids 267-566, epitopes may be conserved across species (cross-reactivity confirmed for human, mouse, rat), but always verify reactivity in your specific sample system.
• Include positive and negative controls, and perform standard blocking and washing steps to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material sourcing, supporting your projects from early R&D through clinical application. For your ubiquitination, neurobiology, or immune signaling studies, we provide validated antibodies like this anti-RNF220 polyclonal, along with antibody pairs, recombinant proteins, and bulk ancillary reagents. Our team also assists with custom antibody generation and rare-target sourcing, ensuring a reliable, end-to-end supply chain for diagnostic manufacturers and research laboratories.