Anti-RNF20 Rabbit Monoclonal Antibody for WB and ELISA - Q5VTR2

Core Product Specifications and Parameters

Biological Background: RNF20 (BRE1A) Function and Localization

• RNF20 (also known as BRE1A) is a RING finger protein that functions as the catalytic subunit of the RNF20/40 E3 ubiquitin-protein ligase complex, specifically mediating monoubiquitination of histone H2B at lysine 120 (H2BK120ub1).
• H2BK120ub1 serves as an epigenetic mark that is prerequisite for histone H3 Lys-4 and Lys-79 methylation (H3K4me and H3K79me), playing a central role in the histone code and gene regulation.
• The RNF20/40 complex cooperates with the E2 enzymes UBE2A or UBE2B to form the histone H2B ubiquitin ligase and is required for transcriptional activation of Hox genes.
• RNF20 acts as a transcriptional coactivator by being recruited to the MDM2 promoter, likely by p53/TP53, and also mediates polyubiquitination of isoform 2 of PA2G4 in cancer cells, leading to its proteasomal degradation.
• The protein localizes exclusively to the nucleus, consistent with its chromatin-regulatory functions.
• RNF20 is expressed in normal brain tissue and malignant gliomas at the protein level, and it is subject to acetylation and phosphorylation as post-translational modifications.
• The protein contains a coiled-coil domain and a zinc-finger motif, enabling metal-binding and typical RING-type ubiquitin transferase activity.
• During microbial infection, RNF20 promotes the human herpesvirus 8 (KSHV) lytic cycle by inducing expression of lytic viral genes, including the latency switch gene RTA/ORF50.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider enriching nuclear fractions due to the nuclear localization of RNF20 to enhance detection sensitivity.
• Validate antibody specificity using knockdown or knockout controls in the relevant cell line or tissue system.
• The synthetic peptide immunogen (aa 1-100 of human RNF20) can be used as a positive control in ELISA assays to confirm antibody reactivity.
• Given cross-reactivity with both human and mouse, the antibody may be suitable for studies in mouse models, but titration and validation in the specific sample type are advised.
• Since RNF20 undergoes phosphorylation and acetylation, additional bands may appear under certain conditions; phosphatase or deacetylase treatments can help confirm band identity.

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CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibodies for epigenetic targets like RNF20. Beyond this product, we offer tailored sourcing for antibody pairs, optimized polyclonal and monoclonal reagents, bulk ancillary materials, and rare-target raw materials to streamline your assay development from concept to clinic. Let us be your procurement bridge for high-quality, research-use antibodies and ancillary reagents in the epigenetics and oncology fields.