Anti-RNF185 Rabbit Polyclonal Antibody for WB and ELISA - Q96GF1

Core Product Specifications and Parameters

Biological Background: RNF185 Function and Localization

• RNF185 (RING finger protein 185) is an E3 ubiquitin-protein ligase characterized by the presence of a RING-type zinc finger domain, which mediates its catalytic transferase activity.
• It mediates 'Lys-63'-linked polyubiquitination of BNIP1, a key step in the regulation of selective mitochondrial autophagy (mitophagy). Related references: PMID:21931693
• Acting in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, RNF185 targets misfolded proteins for ubiquitination and subsequent proteasome-mediated degradation, thereby protecting cells from ER stress-induced apoptosis. Related references: PMID:27485036
• It is responsible for the cotranslational ubiquitination and degradation of the cystic fibrosis transmembrane conductance regulator (CFTR) in the ERAD pathway, and preferentially associates with the E2 enzymes UBE2J1 and UBE2J2. Related references: PMID:24019521
• In the innate antiviral response, RNF185 catalyzes 'Lys-27'-linked polyubiquitination of cGAS at residues K173 and K384, enhancing its cyclic GMP-AMP synthase activity. Related references: PMID:28273161
• Subcellular localization studies reveal that RNF185 is anchored to the mitochondrial outer membrane and the endoplasmic reticulum membrane, consistent with its dual roles in mitophagy and ERAD.
• According to UniProt, alternative splicing of RNF185 generates multiple isoforms, which may exhibit distinct functional properties.
• The protein is ubiquitously expressed across human tissues, supporting a broad housekeeping function in cellular homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–130) of human RNF185, which may allow detection of various isoforms and splice variants. Users should validate the antibody's recognition of specific isoforms in their target samples.
• For Western blotting, a primary band can be expected near 20 kDa (predicted molecular weight: 20.5 kDa from UniProt; supplier reports 20 kDa). Due to alternative splicing, additional bands might be observed; optimization of blocking and detection conditions is recommended.
• The antibody has been reported to cross-react with mouse and rat RNF185; however, species-specific validation in the relevant experimental system is advised.
• When used in ELISA, consider employing the recombinant immunogen or native protein extracts as coating antigens, and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, from validated antibody pairs and high-quality polyclonal/monoclonal antibodies to bulk ancillary reagents and rare target sourcing. Our RNF185 antibody supports investigations into mitochondrial quality control, ER stress pathways, and innate immune mechanisms, providing a reliable tool for assay development. With end-to-end technical and consulting support, CamelBio accelerates your journey from concept to clinic.