Anti-RNF181 Rabbit Polyclonal Antibody for WB and ELISA - Q9P0P0

Core Product Specifications and Parameters

Biological Background: RNF181 Function and Localization

• RNF181 is an E3 ubiquitin-protein ligase that contains a RING finger domain, a specialized zinc‑finger motif that binds two zinc ions and mediates protein–protein interactions essential for ubiquitin transfer.
• It accepts ubiquitin from an E2 ubiquitin‑conjugating enzyme and directly transfers it to target substrates, functioning as a key regulator of ubiquitin‑dependent pathways. Related references: PMID:18331836, PMID:24811749
• RNF181 catalyzes the monoubiquitination of the 26S proteasome subunit PSMC2/RPT1, linking it to proteasome regulation and protein quality control. Related references: PMID:24811749
• The RING finger domain classifies RNF181 as a member of the RING‑type E3 ligase family, which requires zinc coordination for catalytic activity.
• RNF181 is widely expressed, with highest transcript levels observed in liver and heart and lower expression in brain and skeletal muscle; it is also detected at the protein level in platelets.
• Post‑translational modifications include phosphorylation (indicated by the phosphoprotein keyword), which may modulate its activity or interactions.
• UniProt keywords highlight its involvement in ubiquitin conjugation (Ubl conjugation pathway), metal binding, and transferase activity, underscoring its multifaceted cellular roles.

Experimental Guidance and Technical Tips

• The immunogen comprises the full‑length human RNF181 sequence (amino acids 1–153), so the polyclonal antibody may recognize multiple epitopes and potential isoforms; consider verifying isoform detection in your specific sample system.
• For Western blotting, a band near 18 kDa is expected; liver and heart lysates (human, mouse, or rat) are good positive controls based on high tissue expression.
• When using ELISA, include a purified recombinant RNF181 protein as a coating antigen to confirm reactivity and optimize assay conditions.
• Cross‑reactivity with human, mouse, and rat has been reported, but always validate the antibody in the species and tissue type relevant to your experiment, as expression levels may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing. For ubiquitin‑pathway targets like RNF181, we provide custom procurement and supply solutions to help accelerate your assay development and biomarker research. Whether you need high‑quality primary antibodies or complete raw‑material kits, CamelBio is your reliable partner from concept to clinic.