Anti-RNF180 Rabbit Polyclonal Antibody for WB and ELISA - Q86T96

Core Product Specifications and Parameters

Biological Background: RNF180 Function and Localization

• RNF180, also known as RINES or RING finger protein 180, is an E3 ubiquitin-protein ligase that mediates the transfer of ubiquitin to substrate proteins.
• It specifically promotes the polyubiquitination and subsequent proteasome-mediated degradation of ZIC2, a transcription factor critical for brain development.
• The protein contains a RING-type zinc-finger domain, characteristic of RING finger E3 ligases, which binds two zinc ions and is essential for its catalytic activity.
• RNF180 localizes predominantly to the endoplasmic reticulum membrane and the nuclear envelope, indicating a role at the interface of the secretory pathway and nuclear compartment.
• Multiple isoforms are produced by alternative splicing, potentially contributing to functional diversity or regulation.
• Additional features include transmembrane helices, phosphorylation sites, and metal-binding properties, consistent with its membrane-associated and enzymatic functions.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human RNF180 (residues 1–416), enabling recognition of multiple epitopes; confirm specificity in your sample system.
• For western blot, a band around 68 kDa is expected; validate using positive controls (e.g., human, mouse, or rat lysates) and consider using a protease inhibitor cocktail to prevent degradation.
• When using this antibody in ELISA, optimize coating and detection conditions; it can be employed as a capture or detection antibody depending on experimental design.
• Cross-reactivity with mouse and rat suggests sequence conservation; verify performance in these species if intended for use in animal models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive platform for sourcing high-quality IVD raw materials such as validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-RNF180 polyclonal antibody supports research into ubiquitin-mediated proteolysis and protein homeostasis, making it a valuable addition to your diagnostic or research workflows. With our one-stop approach, we also assist in rare-target raw-material sourcing and offer technical consulting to accelerate your project from concept to clinic.