Anti-RNF168 Polyclonal Antibody for WB, ELISA - Q8IYW5

Core Product Specifications and Parameters

Biological Background: RNF168 Function and Localization

• E3 ubiquitin-protein ligase that catalyzes monoubiquitination of nucleosomal histone H2A at Lys-13 and Lys-15 (H2AK13Ub and H2AK15Ub), playing a central role in the DNA damage response.
• Acts with UBE2N/UBC13 downstream of RNF8 to amplify histone H2A and H2AX ubiquitination at double-strand breaks, promoting 'Lys-63'-linked ubiquitin chain formation and recruitment of repair proteins TP53BP1 and BRCA1.
• Also recruited to DNA interstrand cross-link (ICL) sites, where it mediates 'Lys-63'-linked ubiquitination of histones H2A and H2AX, facilitating Fanconi anemia (FA) complex assembly and repair.
• Following DNA damage, collaborates with RNF8 to ubiquitinate and degrade JMJD2A/KDM4A, unmasking the H4K20me2 mark to promote TP53BP1 loading at damage sites.
• Involved in class switch recombination in the immune system by regulating double-strand break repair pathways.
• Localizes to the nucleus, where it is recruited to DNA lesions through binding to pre-existing ubiquitinated histones H2A and H2AX.
• Belongs to the RING finger protein family; contains an N-terminal RING domain and C-terminal MIU motifs that mediate ubiquitin binding.
• Classified as a chromatin regulator and DNA repair factor; key biological keywords include metal-binding, phosphoprotein, transferase, and Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300–571 of human RNF168, a region encompassing the C-terminal MIU motifs; this design may favor detection of the full-length protein at ~65kDa.
• For Western blotting, consider testing nuclear extracts from human or mouse cell lines, as RNF168 localizes to the nucleus and its expression may be induced under DNA-damaging conditions.
• ELISA may be used to screen hybridoma supernatants or quantify RNF168 levels; ensure the recombinant immunogen or a relevant peptide is used as coating antigen.
• Since RNF168 functions in ubiquitin-dependent signaling, include appropriate protease and deubiquitinase inhibitors during sample preparation to preserve potential ubiquitinated species.
• Validate antibody specificity and optimal dilution in your own experimental system, as performance can vary with sample type and treatment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For DNA damage and repair research, this anti-RNF168 polyclonal antibody supports your assay development and diagnostic projects. Contact us to discuss custom supply solutions and technical consultation tailored to your workflow.