Anti-RNF166 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96A37

Core Product Specifications and Parameters

Biological Background: RNF166 Function and Localization

• RNF166 is an E3 ubiquitin-protein ligase containing a RING finger domain, also known as RING finger protein 166 or RING-type E3 ubiquitin transferase RNF166.
• It promotes the ubiquitination of substrates such as TRAF3, TRAF6, and SQSTM1, participating in diverse biological processes including interferon production and xenophagy.
• Plays a critical role in RNA virus-induced interferon-beta production by mediating the ubiquitination of TRAF3 and TRAF6, activating innate immune responses. Related references: PMID:26456228
• Facilitates the early recruitment of autophagy adapters to bacteria and mediates Lys-29 and Lys-33-linked ubiquitination of SQSTM1, leading to xenophagic targeting and inhibition of bacterial replication. Related references: PMID:27880896
• RNF166 localizes predominantly to the cytoplasm, where it exerts its ubiquitin ligase activity.
• The protein is characterized by zinc-finger motifs, is involved in the ubiquitin conjugation pathway, and has been identified in proteomic analyses; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1–237, which may allow recognition of multiple isoforms if the region is conserved. Validate specificity in your sample system.
• For western blotting, expected band size is approximately 26 kDa; consider using appropriate positive and negative controls.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions, as the target is cytoplasmic. Counterstaining for nuclei may help confirm localization.
• For ELISA, titrate the antibody and validate using recombinant protein standards to determine suitable working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with integrated access to IVD raw materials, technical services, and consulting, supporting projects from concept to clinic. This anti-RNF166 polyclonal antibody is a valuable tool for investigating ubiquitination-mediated innate immunity and autophagy pathways. As your sourcing partner, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored support for rare target raw-material procurement.