Anti-RNF166 Polyclonal Antibody for WB, IHC-P, ELISA - Q96A37

Core Product Specifications and Parameters

Biological Background: RNF166 Function and Localization

• RNF166 is an E3 ubiquitin-protein ligase that contains a RING finger domain and zinc-binding sites, characteristic of the RING finger family of ubiquitin transferases.
• It mediates both 'Lys-29' and 'Lys-33'-linked polyubiquitination, distinct from the more common 'Lys-48' and 'Lys-63' linkages, targeting substrates for non-degradative signaling or autophagy. Related references: PMID:27880896
• RNF166 promotes RNA virus-induced interferon-beta production by catalyzing the ubiquitination of TRAF3 and TRAF6, key adaptors in innate immune pathways. Related references: PMID:26456228
• It plays a critical role in xenophagy, the selective autophagy of intracellular bacteria, by ubiquitinating the autophagy receptor SQSTM1/p62 and recruiting autophagy adapters. Related references: PMID:27880896
• RNF166 is predominantly localized in the cytoplasm, positioning it at the hub of immune signaling and intracellular quality control.
• The gene undergoes alternative splicing, generating multiple isoforms that may expand its functional repertoire.
• As a RING-type E3 ligase, RNF166 directly transfers ubiquitin from E2 enzymes to specific substrates, thereby determining the cellular fate of target proteins.

Experimental Guidance and Technical Tips

• For Western blotting, the expected molecular weight of RNF166 is approximately 26 kDa. Include a positive control lysate from human, mouse, or rat cell lines known to express RNF166.
• In IHC-P, consider optimizing antigen retrieval conditions and primary antibody dilution on known positive tissue sections, such as human spleen or lymph node, before proceeding to experimental samples.
• For ELISA, the immunogen peptide can serve as a positive control. Given cross-reactivity with mouse and rat, the antibody may be used to quantify RNF166 across these species after validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-RNF166 polyclonal antibody supports research into innate immunity and ubiquitin-mediated autophagy. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialize in sourcing rare-target raw materials to accelerate your assay development.