Anti-RNF152 Rabbit Polyclonal Antibody for WB, ELISA - Q8N8N0

Core Product Specifications and Parameters

Biological Background: RNF152 Function and Localization

• RNF152 is an E3 ubiquitin-protein ligase capable of catalyzing both Lys-63- and Lys-48-linked polyubiquitination, with the latter targeting proteins for proteasomal degradation. Related references: PMID:21203937
• It functions as a key negative regulator of mTORC1 signaling by mediating ubiquitination of RagA/RRAGA, particularly in response to amino acid starvation. Related references: PMID:25936802
• RNF152 also monoubiquitinates RHEB, promoting its association with the TSC-TBC complex and thereby inhibiting mTORC1 activation. Related references: PMID:30514904
• Overexpression of RNF152 induces apoptosis, highlighting its potential role in programmed cell death. Related references: PMID:21203937
• The protein localizes predominantly to the lysosome membrane, consistent with its role in amino acid sensing and mTORC1 regulation.
• As a member of the RING finger protein family, RNF152 contains a zinc-finger domain essential for its E3 ligase activity.
• RNF152 is widely expressed across various human tissues.

Experimental Guidance and Technical Tips

• The immunogen was derived from a synthetic peptide spanning amino acids 100-200 of human RNF152. This region may exhibit favorable immunogenicity; confirm specificity in your relevant sample system.
• This antibody has been validated for WB and ELISA. For applications such as IP or IF, optimization and additional validation are recommended.
• With a predicted protein weight of 22 kDa, ensure appropriate molecular weight markers and loading controls are used in Western blot analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, offering diagnostic manufacturers, research labs, and institutes seamless access to high-quality reagents. This anti-RNF152 antibody supports research into ubiquitin ligases and mTOR signaling pathways. Beyond this product, CamelBio supplies validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist with sourcing rare target raw materials.