Anti-RNF149 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8NC42

Core Product Specifications and Parameters

Biological Background: RNF149 Function and Localization

• Protein Identity and Aliases: E3 ubiquitin-protein ligase RNF149, also designated as DNA polymerase-transactivated protein 2 (DNAPTP2) and RING finger protein 149.
• Core Function: Functions as an E3 ubiquitin-protein ligase that mediates ubiquitination of BRAF, targeting it for proteasomal degradation.
• Protein Domain and Classification: Contains a RING-type zinc finger domain, classifying it as a RING-type E3 ubiquitin transferase involved in the ubiquitin conjugation pathway.
• Subcellular Localization: Predicted to be a membrane protein, possessing a signal peptide and a transmembrane helix that anchors it to cellular membranes.
• Post-translational Modifications: Identified as a glycoprotein and phosphoprotein; zinc ion binding is essential for its RING finger structure and catalytic activity.
• Molecular Weight: The predicted molecular mass is approximately 43 kDa (UniProt: 43,165 Da), in agreement with the observed protein weight.
• Proteomics Identification: Detected at the protein level through proteomic analyses.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 221–400) likely corresponds to a cytoplasmic region; consider whether the epitope is accessible in your sample preparation (e.g., denaturing versus native conditions for WB/IHC).
• For Western blot, expect a band near 43 kDa. Use appropriate positive control lysates from human, mouse, or rat cell lines.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), perform antigen retrieval as needed and include negative controls (e.g., peptide blocking or knockout cells) to confirm specificity.
• Because this is a rabbit polyclonal antibody, lot-to-lot variability may occur. Titrate the antibody for each application and validate in your experimental system.
• For ELISA, the antibody can be used for detection; consider pairing it with a validated monoclonal capture antibody if developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, supporting researchers and diagnostic developers from early research through to commercialization. This anti-RNF149 polyclonal antibody is part of our portfolio for ubiquitination and cancer signaling studies. We complement our catalog with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets, helping you streamline assay development and production.