Anti-RNF149 Polyclonal Antibody for IF/ICC, ELISA - Q8NC42

Core Product Specifications and Parameters

Biological Background: RNF149 Function and Localization

• RNF149 (DNA polymerase-transactivated protein 2) is a RING finger protein and E3 ubiquitin-protein ligase, classified as a RING-type E3 ubiquitin transferase.
• It ubiquitinates BRAF, targeting it for proteasomal degradation, thereby regulating BRAF protein levels and downstream signaling.
• The protein contains a RING finger domain responsible for its E3 ligase activity and a transmembrane domain, belonging to a family of membrane-bound E3 ligases.
• Subcellularly, RNF149 is localized to the membrane; it is a transmembrane protein with a signal sequence.
• Post-translational modifications include glycosylation and phosphorylation, and it binds zinc as a zinc-finger protein, indicating structural and regulatory features.
• RNF149 plays a role in the ubiquitin conjugation pathway, influencing protein turnover and cellular homeostasis.
• Keywords associated with RNF149 include Glycoprotein, Membrane, Metal-binding, Phosphoprotein, Transferase, and Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide from amino acids 200-300 of human RNF149; for IF/ICC, consider optimizing fixation and permeabilization protocols due to the membrane localization of the target.
• When using this antibody in ELISA, experimental determination of optimal working concentrations and inclusion of appropriate positive/negative controls are recommended.
• Cross-reactivity with rat has been reported; verify performance in rat models by conducting initial validation experiments.
• Since RNF149 is a membrane protein, denaturing conditions may not fully expose all epitopes; consider antigen retrieval or native conditions if issues arise.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, offering validated antibodies like this anti-RNF149 polyclonal antibody to support research into ubiquitination, BRAF degradation, and membrane-associated E3 ligase pathways. Our platform provides bulk quantities, customized monoclonal/polyclonal antibody development, and ancillary reagents, streamlining procurement for diagnostic and research laboratories. For niche targets or rare raw materials, we bridge supply gaps to accelerate your project from concept to clinic.