Anti-RNF144A Polyclonal Antibody for WB, ELISA - P50876

Core Product Specifications and Parameters

Biological Background: RNF144A Function and Localization

• RNF144A, also known as UIP4, RNF144, or UBCE7IP4, is an E3 ubiquitin-protein ligase containing a RING finger domain and a transmembrane helix.
• It accepts ubiquitin from E2 enzymes UBE2L3 and UBE2L6 and directly transfers it to substrate proteins, functioning as a key component of the ubiquitination machinery. Related references: PMID:26216882
• During DNA damage, RNF144A mediates the ubiquitination and degradation of the DNA damage kinase PRKDC, contributing to DNA repair regulation. Related references: PMID:24979766
• It positively regulates innate immune responses by mediating STING1 ubiquitination and promoting its translocation from the endoplasmic reticulum to the Golgi, thereby activating downstream signaling pathways. Related references: PMID:37955227
• RNF144A plays a positive role in EGF-dependent cell proliferation by prolonging EGFR signaling through EGFR ubiquitination. Related references: PMID:30171075
• Independently of EGFR, it increases ERK activity by promoting polyubiquitination and subsequent degradation of VRK3 in the cytosol. Related references: PMID:33067254
• Subcellularly, RNF144A is found at the cell membrane, cytoplasmic vesicle membrane, endosome membrane, and endoplasmic reticulum membrane.
• The protein features a zinc-finger RING-type domain essential for its ligase activity and a transmembrane region anchoring it to membranes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 52-179 of human RNF144A, targeting the N-terminal region; this region is present in the full-length protein and may detect isoforms.
• For Western blot, a band around 33 kDa is expected in human and rat lysates; validate molecular weight in your specific sample system and consider using loading controls.
• In ELISA applications, optimize coating antigen concentration and antibody dilution; the polyclonal nature may provide broad epitope recognition suitable for sandwich assay development.
• Confirm specificity by using RNF144A knockout or knockdown cell lines, as the antibody may cross-react with related RING finger proteins.
• For immunohistochemistry or other untested applications, pilot experiments are recommended to determine suitability.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio offers one-stop access to high-quality antibodies, validated antibody pairs, and bulk ancillary reagents for diagnostic and research applications. The RNF144A antibody supports studies in ubiquitination, DNA damage response, and innate immunity, and we can assist in sourcing complementary reagents such as detection antibodies and recombinant proteins. Contact us to explore customized monoclonal antibody development, bulk production, or rare target raw-material sourcing tailored to your assay development needs.