Anti-RNF14 Monoclonal Antibody for WB, ELISA - Q9UBS8

Core Product Specifications and Parameters

Biological Background: RNF14 Function and Localization

• Also known as Androgen receptor-associated protein 54 (ARA54), HFB30, and RING finger protein 14. Multiple isoforms exist due to alternative splicing.
• Functions as an E3 ubiquitin-protein ligase in the RNF14-RNF25 translation quality control pathway, mediating 'Lys-6'-linked ubiquitination of translation factors (EEF1A1, ETF1) and ribosomal proteins on stalled ribosomes (PubMed:36638793, PubMed:37651229, PubMed:37951215). Related references: PMID:36638793, PMID:37651229, PMID:37951215
• Required for resolving RNA-protein cross-links caused by reactive aldehydes, promoting degradation of cross-linked products via the VCP/proteasome pathway (PubMed:37951215, PubMed:37951216). Related references: PMID:37951215, PMID:37951216
• Acts as a transcriptional regulator in Wnt signaling through interaction with TCF transcription factors TCF7/TCF1, TCF7L1/TCF3, and TCF7L2/TCF4 (PubMed:23449499). Related references: PMID:23449499
• May function as a coactivator for androgen- and progesterone-dependent transcription (PubMed:19345326). Related references: PMID:19345326
• Subcellular localization includes both cytoplasm and nucleus.
• Widely expressed across tissues.
• Contains zinc-finger domains and is subject to phosphorylation; classified under transferase, Ubl conjugation pathway, and metal-binding proteins.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the internal region (aa 300–400) of human RNF14; this may favor recognition of denatured linear epitopes, making the antibody well-suited for Western blotting (WB) under reducing/denaturing conditions.
• For ELISA applications, consider using the immunizing peptide as a positive coating antigen and validate the antibody's performance with full-length recombinant protein or cell lysates from human, mouse, or rat sources to confirm cross-reactivity.
• Since RNF14 is expressed in both cytoplasm and nucleus, ensure appropriate fractionation or total lysate preparation to detect the target in WB.
• Verify antibody specificity by testing in RNF14-overexpressing or knockdown cell lines, if available.
• Use appropriate loading controls (~54 kDa) and confirm the observed band size matches the predicted molecular weight of RNF14.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-RNF14 antibody supports research into translation quality control, Wnt signaling, and androgen receptor coactivation, areas with growing diagnostic potential. Contact us to streamline your assay development from discovery to clinical application.