Anti-RNF126 Polyclonal Antibody for WB, IHC-P, ELISA - Q9BV68

Core Product Specifications and Parameters

Biological Background: RNF126 Function and Localization

• RNF126 is an E3 ubiquitin-protein ligase that catalyzes the ubiquitination of target proteins, mediating both degradative and non-degradative polyubiquitin chains depending on the associated E2 enzyme.
  Related references:
  PMID:23277564
  PMID:24275455
  PMID:24981174

• As a component of the BAG6 complex, RNF126 ensures the proteasomal degradation of secretory pathway proteins mislocalized to the cytosol by providing the ubiquitin ligase activity.
  Related references:
  PMID:24981174
  PMID:29042515

• RNF126 plays a role in the endosomal sorting and degradation of several membrane receptors, including EGFR, FLT3, MET, and CXCR4, through ubiquitination.
  Related references:
  PMID:23418353

• It promotes cell proliferation by ubiquitinating the cyclin-dependent kinase inhibitor CDKN1A/p21, targeting it for degradation.
  Related references:
  PMID:23026136

• RNF126 regulates DNA repair by mediating 'Lys-27'- and 'Lys-29'-linked polyubiquitination of MRE11, thereby enhancing its exonuclease activity.
  Related references:
  PMID:36563124

• The protein localizes to both the cytoplasm and nucleus.

• RNF126 is highly expressed in liver and testis tissues.

• It contains a RING finger domain that coordinates zinc ions, characteristic of many E3 ligases, and undergoes post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from liver or testis, which highly express RNF126, as positive controls. Validate the antibody in your specific sample system and optimize blocking and detection conditions.
• In immunohistochemistry (IHC-P), antigen retrieval may be required; test various retrieval buffers and validate in the relevant tissue samples.
• For ELISA applications, this polyclonal antibody can be used as a capture or detection reagent after proper pairing and optimization with monoclonal antibodies or other detection reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies like anti-RNF126, and bulk ancillary reagents to accelerate research in ubiquitination, protein quality control, and DNA repair. With expertise in rare-target sourcing, CamelBio is your trusted partner for building robust diagnostics.