Anti-RNF125 Rabbit Polyclonal Antibody for WB and ELISA - Q96EQ8

Core Product Specifications and Parameters

Biological Background: RNF125 Function and Localization

• Functions as an E3 ubiquitin-protein ligase, catalyzing the ubiquitination and proteasomal degradation of key signaling molecules including RIGI, MAVS/IPS1, IFIH1/MDA5, JAK1, and p53/TP53 (PubMed:15843525, 17460044, 17643463). Related references: PMID:15843525 PMID:17460044 PMID:17643463

• Acts as a negative regulator of type I interferon production by specifically ubiquitinating RIGI at Lys-181, targeting it for degradation (PubMed:17460044, 26471729). Related references: PMID:17460044 PMID:26471729

• Plays a positive role in T-cell activation (PubMed:15843525). Related references: PMID:15843525

• Localizes predominantly to the Golgi apparatus membrane.

• Expressed mainly in lymphoid tissues—bone marrow, spleen, thymus—and enriched in CD4+ and CD8+ T-cells.

• Contains a RING-type zinc finger domain essential for ubiquitin ligase activity; also possesses myristoylation and lipidation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-105) of human RNF125; consider this when designing detection strategies.
• Validated for Western blot and ELISA; users should optimize antibody dilution and validate in their specific sample system, particularly lymphoid-derived cell lines or T-cell extracts.
• Observed protein weight is approximately 26 kDa; a band at this size is expected in reducing SDS-PAGE of human or mouse samples.
• Given its localization to Golgi, membrane fractionation may improve detection in certain assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-RNF125 polyclonal antibody is part of our portfolio for innate immunity and oncology research, backed by validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate your assay development. For rare target sourcing or bulk orders, our technical team provides tailored procurement support.