Anti-RNF125 Polyclonal Antibody for WB, ELISA - Q96EQ8

Core Product Specifications and Parameters

Biological Background: RNF125 Function and Localization

• RNF125, also known as TRAC-1 (T-cell RING activation protein 1), is an E3 ubiquitin-protein ligase containing a RING finger domain.
• It mediates ubiquitination and proteasomal degradation of multiple signaling proteins, including RIGI, MAVS, IFIH1, JAK1, and p53, thereby regulating innate immunity, apoptosis, and T-cell activation.
  Related references: PMID:15843525 PMID:17460044 PMID:17643463 PMID:25591766 PMID:26027934
• Functions as a negative regulator of type I interferon production by targeting RIGI for ubiquitin-dependent degradation.
  Related references: PMID:17460044 PMID:26471729
• Promotes ubiquitination and subsequent degradation of p53/TP53, influencing cell cycle arrest and apoptosis.
  Related references: PMID:25591766
• Mediates degradation of JAK1, thereby modulating JAK-STAT signal transduction.
  Related references: PMID:26027934
• Acts as a positive regulator of T-cell activation and is predominantly expressed in lymphoid tissues, especially CD4+ and CD8+ T-cells.
  Related references: PMID:15843525
• Localized to the Golgi apparatus membrane, with myristoylation likely contributing to membrane anchoring.
• Genetic variants of RNF125 have been associated with intellectual disability.
• Keywords: 3D-structure, adaptive immunity, disease variant, Golgi apparatus, intellectual disability, membrane, metal-binding, myristate, transferase, Ubl conjugation, zinc-finger.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 41-200 of human RNF125, covering the N-terminal region upstream of the RING domain. For Western blot (WB), consider using reducing conditions and validate the antibody in human T-cell lines (e.g., Jurkat) or lymphoid tissues where RNF125 expression is highest.
• For ELISA applications, optimize coating antigen and antibody dilutions using recombinant RNF125 protein or cell lysates from RNF125-overexpressing systems.
• As a polyclonal antibody, batch-to-batch variability may occur; it is recommended to validate each new lot in the intended assay with appropriate controls.
• Cross-reactivity with other species has not been tested; verify specificity if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including polyclonal antibodies like this anti-RNF125 product for ubiquitination and immunology research. Our portfolio encompasses validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing capabilities to support your assay development and clinical applications from concept to clinic.