Anti-RNF115 Polyclonal Antibody for WB, ELISA - Q9Y4L5

Core Product Specifications and Parameters

Biological Background: RNF115 Function and Localization

• RNF115 (also known as BCA2, ZNF364, Rabring7) is a RING finger-containing E3 ubiquitin-protein ligase that mediates both 'Lys-48'- and 'Lys-63'-linked polyubiquitination of substrate proteins, regulating diverse processes including autophagy, innate immunity, and cell proliferation (PubMed:20019814, PubMed:30689267). Related references: PMID:20019814, PMID:30689267
• It plays a critical role in endosomal trafficking and lysosomal degradation of membrane receptors such as EGFR, FLT3, MET, and CXCR4 by promoting their ubiquitination.
• RNF115 participates in antiviral defense by facilitating BST2-dependent internalization of HIV-1 virions and BST2-independent ubiquitination of retroviral GAG proteins, targeting them for lysosomal degradation (PubMed:20019814, PubMed:24852021). Related references: PMID:20019814, PMID:24852021
• During viral infection, RNF115 dynamically ubiquitinates MAVS and STING1: it catalyzes 'Lys-48'-linked ubiquitination of MAVS for proteasomal degradation and 'Lys-63'-linked polyubiquitination of STING1 to promote its oligomerization and TBK1 recruitment (PubMed:33139700). Related references: PMID:33139700
• The protein positively regulates autophagosome-lysosome fusion by interacting with STX17 and enhancing its stability, thereby promoting autophagosome maturation (PubMed:32980859). Related references: PMID:32980859
• RNF115 negatively regulates TLR-induced proinflammatory cytokines by catalyzing 'Lys-11'-linked ubiquitination of RAB1A and RAB13, inhibiting post-ER trafficking of TLRs (PubMed:35343654). Related references: PMID:35343654
• Subcellularly, RNF115 localizes to the cytoplasm, nucleus, endoplasmic reticulum, and Golgi apparatus. It is expressed at very low levels in normal breast, prostate, lung, and colon but shows higher expression in heart, skeletal muscle, testis, and various cancer cells.
• Post-translational modifications include acetylation and phosphorylation, and the protein contains a zinc-finger domain essential for its E3 ligase activity.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to amino acids 30-140 of human RNF115, which lies within the N-terminal region. This may generate antibodies targeting epitopes in this domain.
• As a rabbit polyclonal antibody, it is expected to recognize multiple epitopes, potentially offering robust detection but with inherent lot-to-lot variability. Researchers should validate each new lot in their specific assay conditions.
• The antibody is suitable for Western blotting (WB) and ELISA applications. For WB, consider using a positive control lysate from human cell lines or rat tissues (given the reported cross-reactivity), and confirm the observed band at approximately 34 kDa.
• Cross-reactivity with rat is indicated; however, performance in other species should be validated experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop partner for IVD manufacturers and research laboratories, offering streamlined access to high-quality raw materials and technical support for ubiquitin signaling, autophagy, and innate immunity studies. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare targets like RNF115 to accelerate your assay development. Our integrated services cover every stage from concept to clinic, ensuring you have the reliable components needed for robust diagnostic and research applications.