Anti-RNF114 Polyclonal Antibody for WB, ELISA - Q9Y508

Core Product Specifications and Parameters

Biological Background: RNF114 Function and Localization

• RNF114 is a RING finger protein that functions as an E3 ubiquitin-protein ligase, targeting substrates for ubiquitination and proteasomal degradation. Related references: PMID:23645206, PMID:25165885
• It negatively regulates NF-kappa-B signaling by ubiquitinating and stabilizing the inhibitor TNFAIP3, and may also target TRAF6. Related references: PMID:25165885, PMID:28708287
• In T cells, RNF114 acts as a negative regulator of activation, helping to modulate adaptive immune responses. Related references: PMID:25165885
• RNF114 inhibits innate antiviral responses by promoting degradation of the mitochondrial antiviral-signaling protein MAVS, suppressing interferon production. Related references: PMID:25165885
• It regulates the cell cycle by ubiquitinating the CDK inhibitor p21 (CDKN1A), leading to its degradation and promoting G1-to-S phase transition, thus suppressing cellular senescence. Related references: PMID:23645206
• The protein localizes to both the cytoplasm and nucleus, consistent with its multifunctional roles.
• Highest expression is observed in the testis, suggesting a role in spermatogenesis, and it is also expressed in skin, CD4 lymphocytes, and dendritic cells.
• Keywords include acetylation, alternative splicing, metal-binding zinc finger, and involvement in the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• For Western blot, optimize the primary antibody dilution based on your sample type; the predicted molecular weight is ~26 kDa.
• For ELISA, confirm coating antigen compatibility; the immunogen (amino acids 1-228) can serve as a positive control if needed.
• Since this antibody recognizes human and mouse RNF114, validate species cross-reactivity in your experimental model.
• As a polyclonal antibody, it may detect multiple isoforms or post-translationally modified forms; use appropriate knockdown or knockout controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a centralized platform for IVD raw-material procurement, providing high-quality RNF114 antibodies and related reagents for ubiquitin research. Our catalog includes validated polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents to support your assay development and diagnostic manufacturing needs. With expert technical consulting and a global supply network, we help you source even rare targets efficiently.