Anti-RNF10 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8N5U6

Core Product Specifications and Parameters

Biological Background: RNF10 Function and Localization

• RNF10 (RING finger protein 10, also known as RIE2) is an E3 ubiquitin-protein ligase that plays a critical role in ribosome quality control.
• It catalyzes monoubiquitination of 40S ribosomal proteins RPS2 and RPS3 in response to ribosome stalling during translation initiation (iRQC) or elongation. Related references: PMID:34348161, PMID:34469731, PMID:39609413
• RNF10 activity is triggered by ribosome subunit imbalance, amino acid starvation, or downstream of the EIF2AK4/GCN2-mediated integrated stress response (ISR). Related references: PMID:39609413, PMID:39947182, PMID:39947183
• Monoubiquitinated RPS2 and RPS3 are recognized by RIOK3 kinase, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit. Related references: PMID:34348161, PMID:34469731, PMID:39609413
• The action of RNF10 in iRQC is counteracted by the deubiquitinase USP10. Related references: PMID:34469731
• RNF10 localizes to both the cytoplasm and nucleus.
• It contains a RING-type zinc finger domain and metal-binding motifs, characteristic of E3 ubiquitin ligases.
• Additional features include alternative splicing, phosphorylation, and involvement in the ubiquitin-conjugation pathway (Keywords: Alternative splicing, Cytoplasm, Metal-binding, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Transferase, Ubl conjugation pathway, Zinc, Zinc-finger).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 660–760 of human RNF10 (NP_055683.3), which may confer specificity across human, mouse, and rat targets.
• For Western blotting (WB), the predicted molecular weight is approximately 90 kDa; consider running appropriate controls and validating band sizes in your sample system.
• In immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), antigen retrieval and fixation conditions should be optimized, and note the cytoplasmic/nuclear localization of RNF10.
• For ELISA applications, the antibody may be used for capture or detection; validate linearity and sensitivity in your assay buffer.
• Cross-reactivity with other RING finger proteins is unlikely but should be assessed if non-specific signals are observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including this RNF10 rabbit monoclonal antibody for ribosome quality control and ubiquitination studies. Our supply programs cover validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—supporting your transition from concept to clinic. Reach out to leverage our B2B procurement channels for your next diagnostic development.