Anti-RNF10 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8N5U6

Core Product Specifications and Parameters

Biological Background: RNF10 Function and Localization

• RNF10 is an E3 ubiquitin-protein ligase, also known as RING finger protein 10, encoded by the gene RNF10 (KIAA0262/RIE2). Human RNF10 has a predicted molecular weight of ~90 kDa and undergoes alternative splicing.
• It specifically catalyzes monoubiquitination of 40S ribosomal proteins RPS2/us5 and RPS3/us3 in response to ribosome stalling during translation initiation or elongation. Related references: PMID:34348161 PMID:34469731 PMID:39609413
• RNF10 is a key component of the ribosome quality control (RQC) pathway that is activated under conditions of ribosomal subunit imbalance, amino acid starvation, or downstream of the integrated stress response (ISR) via EIF2AK4/GCN2. Related references: PMID:39609413 PMID:39947182 PMID:39947183
• Monoubiquitinated RPS2 and RPS3 are recognized by RIOK3 kinase, which triggers 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit. Related references: PMID:34348161 PMID:34469731 PMID:39609413
• The ubiquitin ligase activity of RNF10 is counterbalanced by the deubiquitinase USP10, which removes ubiquitin from RPS2 and RPS3. Related references: PMID:34469731
• RNF10 localizes to both the cytoplasm and the nucleus, consistent with its role in ribosome surveillance across these compartments.
• RNF10 contains a RING-type zinc finger domain and is classified as a metal-binding phosphoprotein involved in the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 660–760) of human RNF10, which may confer good specificity for detection by WB, IF/ICC, and ELISA.
• Validate the antibody in relevant species (human, mouse, rat) and check for a band at ~89–90 kDa to confirm recognition of full-length RNF10.
• For IF/ICC, consider optimizing fixation and permeabilization conditions to ensure proper antigen accessibility.
• Since RNF10 participates in stress-activated pathways, induction of the integrated stress response (e.g., by amino acid starvation) may alter protein levels or localization, which could be useful in experimental design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-RNF10 polyclonal antibody supports investigations into ribosome quality control and ubiquitin-mediated proteostasis, and our team can assist with sourcing rare targets or customizing reagent panels for your assay development pipeline. Whether you need single antibodies or full reagent sets, CamelBio is your trusted bridge from concept to clinic.