Anti-RND1 Rabbit Polyclonal Antibody for WB - Q92730

Core Product Specifications and Parameters

Biological Background: RND1 Function and Localization

• RND1 (Rho family GTPase 1) is a member of the Rho family of small GTPases, functioning as a molecular switch in actin cytoskeleton remodeling.
• It lacks intrinsic GTPase activity and has low affinity for GDP, resulting in constitutive GTP binding and active signaling.
• RND1 induces Rac-dependent neuritic process formation and disrupts cortical actin filaments, playing a key role in neuronal morphology.
• Subcellularly, RND1 localizes to the cell membrane, cytoplasm, and cytoskeleton, consistent with its role in actin dynamics.
• The protein undergoes post-translational modifications including prenylation and methylation, which facilitate membrane association.
• Tissue expression is highest in the brain and liver, suggesting specialized roles in these organs.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 133–232, so the antibody may recognize the C-terminal region of RND1; consider epitope mapping for critical applications.
• For Western blotting, the predicted molecular weight is 26 kDa; a 12–15% SDS-PAGE gel and appropriate loading controls are recommended.
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions due to the protein's membrane and cytoskeletal localization.
• The antibody cross-reacts with mouse and rat; validate in the relevant species model before use.
• As a polyclonal antibody, verify lot-to-lot consistency if used in quantitative or regulated assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-RND1 polyclonal antibody supports investigations into actin remodeling and neuronal development. For rare-target sourcing, custom immunogen design, or large-volume procurement, our team ensures seamless supply chain support across the full development pipeline.