Anti-RMND5A Rabbit Polyclonal Antibody for WB, ELISA - Q9H871

Core Product Specifications and Parameters

Biological Background: RMND5A Function and Localization

• RMND5A is an E3 ubiquitin-protein transferase, also known as p44CTLH, Protein RMD5 homolog A, and GID2.
• It is a core component of the CTLH E3 ubiquitin-protein ligase complex, which selectively accepts ubiquitin from UBE2H and mediates ubiquitination and proteasomal degradation of the transcription factor HBP1.
• Both RMND5A and MAEA are required for the catalytic activity of the CTLH E3 ubiquitin-protein ligase complex, and this activity is necessary for normal cell proliferation. Related references: PMID:29911972
• The CTLH complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1.
• RMND5A localizes to the nucleus (nucleoplasm) and cytoplasm.
• It contains a zinc-finger domain and is involved in metal binding, as indicated by UniProt keywords.
• Post-translational modifications include acetylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a dilution range appropriate for polyclonal antibodies and validate with positive controls from human, mouse, or rat cell lysates to confirm specificity.
• In ELISA, the immunogen derived from amino acids 200-270 may be used to detect RMND5A; however, optimization of coating and detection conditions is recommended.
• Given the predicted cross-reactivity with mouse and rat, the antibody may recognize conserved epitopes; validation in the relevant species is advised.
• The polyclonal nature of the antibody may provide robust signal, but batch-to-batch consistency should be monitored.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw material sourcing, delivering a wide array of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-RMND5A polyclonal antibody is designed to support research into ubiquitin-proteasome pathways, and our team is equipped to assist with sourcing rare targets and custom antibody development to accelerate your diagnostic and research endeavors.