Anti-RMI2 Rabbit Polyclonal Antibody for WB, ELISA - Q96E14

Core Product Specifications and Parameters

Biological Background: RMI2 Function and Localization

• RMI2 (RecQ-mediated genome instability protein 2), also known as BLAP18 (BLM-associated protein of 18 kDa), is encoded by the RMI2 (C16orf75) gene.
• It is an essential component of the RMI complex, which plays a critical role in processing homologous recombination intermediates, regulating sister chromatid segregation, and limiting DNA crossover.
• RMI2 is required for the stability, localization, and function of Bloom syndrome helicase (BLM) and topoisomerase III alpha (TOP3A), as well as complexes containing these proteins.
• Within the RMI complex, RMI2 targets BLM to chromatin and stress-induced nuclear foci, and is necessary for mitotic phosphorylation of BLM.
• RMI2 localizes to the nucleus, consistent with its role in DNA repair and replication.
• Post-translational modifications of RMI2 include phosphorylation and acetylation, which may regulate its function.
• According to UniProt, additional keywords include DNA replication, DNA-binding, and alternative splicing, reflecting its involvement in genomic maintenance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-147 of human RMI2, covering the full-length protein. For Western blotting, use whole-cell lysates from human cell lines; consider siRNA-mediated knockdown or overexpression controls to confirm specificity.
• RMI2 has a predicted molecular weight of ~16 kDa; optimize SDS-PAGE with high-percentage gels and low-molecular-weight transfer conditions.
• For ELISA applications, titrate the antibody against recombinant RMI2 protein to determine optimal coating and detection concentrations.
• Due to RMI2’s nuclear localization, nuclear extraction protocols may improve detection in subcellular fractionation studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our team specializes in sourcing rare targets and custom reagent requirements, supporting your DNA repair and genomic stability research with high-quality materials. Whether you need this anti-RMI2 antibody or other components for nucleic acid assays, CamelBio is your reliable partner from concept to clinic.