Anti-RMI1 Rabbit Polyclonal Antibody for WB and ELISA - Q9H9A7

Core Product Specifications and Parameters

Biological Background: RMI1 Function and Localization

• RecQ-mediated genome instability protein 1 (RMI1), also known as BLAP75 or FAAP75, is an essential component of the RMI complex that limits DNA crossover formation during homologous recombination.
• Within the RMI complex, RMI1 promotes the binding of topoisomerase III alpha (TOP3A) to double Holliday junctions, stimulating TOP3A-mediated dissolution of these recombination intermediates.
• RMI1 is required for BLM phosphorylation during mitosis, a critical event for maintaining genomic stability.
• In the BLM complex, RMI1 contributes to the stability of both BLM and TOP3A proteins.
• The protein localizes predominantly in the nucleus, consistent with its role in DNA replication and repair.
• Keywords associated with RMI1 include DNA replication, phosphoprotein, acetylation, and ubiquitin conjugation, indicating post-translational modifications that may regulate its function.

Experimental Guidance and Technical Tips

• The immunogen used for generating this polyclonal antibody covers the N-terminal region (aa 1-350) of human RMI1, which may be suitable for detecting both full-length and N-terminal fragments.
• For Western blot (WB) applications, a specific band around 70 kDa is expected; optimization of blocking and detection conditions is recommended to minimize cross-reactivity.
• The antibody cross-reacts with both human and mouse RMI1, as per supplier specifications; verify in your specific experimental setup.
• In ELISA workflows, titration of primary and secondary antibodies under standardized conditions can enhance signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by providing a reliable supply of validated antibodies, including this anti-RMI1 polyclonal antibody for DNA repair research. Our portfolio covers monoclonal and polyclonal formats, antigen pairs, and ancillary reagents, enabling seamless integration into diagnostic assay development. For rare targets and custom sourcing, CamelBio offers tailored solutions to bridge concept-to-clinic demands.