Anti-RLN2 Polyclonal Antibody for WB, IHC-P, ELISA - P04090

Core Product Specifications and Parameters

Biological Background: RLN2 Function and Localization

• Prorelaxin H2, encoded by the RLN2 gene, belongs to the relaxin family of peptide hormones and serves as a key regulator in reproductive biology.
• It functions as an ovarian hormone that, together with estrogen, promotes the dilation of the birth canal in mammals.
• The protein is involved in connective tissue remodeling during pregnancy, including the growth of pubic ligaments and ripening of the cervix.
• Alternative splicing generates multiple isoforms: isoform 1 is predominantly expressed in the ovary during pregnancy, while isoform 2 is relatively abundant in placenta.
• RLN2 is a secreted protein, consistent with its role as a circulating hormone.
• Beyond the ovary, it is also detected in placenta, decidua, and prostate, reflecting its broader physiological roles.
• Post-translational processing includes cleavage on a pair of basic residues and formation of pyrrolidone carboxylic acid, along with essential disulfide bonds.
• The mature hormone is derived from prorelaxin, and its three-dimensional structures have been experimentally determined.

Experimental Guidance and Technical Tips

• For western blot analysis, a band at approximately 21 kDa may be observed; pre-incubation with the immunogen can help confirm band specificity.
• In immunohistochemistry (IHC-P), consider using placental or prostate tissues as positive controls, with appropriate negative controls to assess staining specificity.
• As a polyclonal antibody, it may recognize multiple RLN2 isoforms; validate detection profiles in your specific sample types.
• For ELISA applications, the antibody may be utilized as a capture or detection reagent; however, optimization of coating concentration and buffer conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials, including this RLN2 polyclonal antibody for reproductive biology studies. With offerings ranging from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and custom sourcing for rare targets, CamelBio bridges the gap from concept to clinic. Our technical expertise supports consistent reagent quality and reliable supply chains for your reproductive health and pregnancy-related research needs.