Anti-RIPK4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P57078

Core Product Specifications and Parameters

Biological Background: RIPK4 Function and Localization

• Encoded by the RIPK4 gene (also known as ANKRD3, DIK), Receptor-interacting serine/threonine-protein kinase 4 is a member of the RIP kinase family, involved in signal transduction.
• Functions as a serine/threonine protein kinase required for embryonic skin development and adult skin homeostasis; it phosphorylates PKP1 to promote keratinocyte differentiation and cell adhesion (By similarity).
• Acts as a direct transcriptional target of TP63, linking epithelial differentiation to signaling cascades. Related references: PMID:22197488
• Plays a role in NF-kappa-B activation, contributing to inflammatory and immune responses. Related references: PMID:12446564
• Localizes to the cytoplasm and membrane, consistent with its role in membrane-proximal signaling.
• Expressed in hair follicles and skin, aligning with its critical function in epidermal biology.
• Contains ankyrin repeat domains and a kinase domain, and is subject to post-translational modifications, including phosphorylation and ubiquitination (see Keywords: Alternative splicing, ANK repeat, ATP-binding, Isopeptide bond, Phosphoprotein, Ubl conjugation).
• Mutations in RIPK4 are associated with ectodermal dysplasia and related disorders, highlighting its clinical significance (Disease variant keyword).

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 240-520 of human RIPK4, which covers the kinase domain and part of the ankyrin repeat region, ensuring reactivity with multiple isoforms.
• For Western blotting, a band at approximately 92 kDa is expected. Use appropriate controls and consider the observed molecular weight may differ slightly due to post-translational modifications.
• IHC-P application may require antigen retrieval; validate in positive control tissues such as skin or hair follicle-containing sections, given the expression pattern.
• Cross-reactivity with mouse makes this antibody suitable for translational studies in murine models of skin development and inflammation.
• For ELISA, consider paired antibody formats for quantitative detection; optimal dilution factors should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with seamless access to high-quality IVD raw materials. For your skin development and inflammation studies, we supply validated anti-RIPK4 antibodies and can assemble complete reagent panels including secondary detection components. Our global sourcing network ensures reliable delivery of even rare target materials, accelerating your assay development from concept to clinic.