Anti-RIPK3 (RIP3) Monoclonal Antibody for WB, IHC-P, ELISA - Q9Y572

Core Product Specifications and Parameters

Biological Background: RIPK3 (Receptor-interacting serine/threonine-protein kinase 3) Function and Localization

• Receptor-interacting serine/threonine-protein kinase 3 (RIPK3), also known as RIP3, is a key mediator of programmed necrosis (necroptosis) and apoptosis, functioning downstream of death receptors and pathogen sensors. Related references: PMID:19524512 PMID:19524513 PMID:22265413
• Upon activation by ZBP1 or TNF receptor signaling, RIPK3 forms a necrosis-inducing complex and phosphorylates MLKL, triggering its translocation to the plasma membrane, calcium influx, and membrane rupture. Related references: PMID:19524512 PMID:19524513 PMID:22265413
• RIPK3 also regulates apoptosis through a RIPK1-, FADD-, and CASP8-dependent pathway that is independent of MLKL and its own kinase activity.
• The protein is found in the cytosol and can translocate to the nucleus, where it mediates necroptosis in response to viral infection by disrupting the nuclear envelope.
• RIPK3 binds and enhances the activity of metabolic enzymes GLUL, GLUD1, and PYGL, potentially stimulating the TCA cycle and oxidative phosphorylation to increase ROS production. Related references: PMID:19498109
• Highly expressed in the pancreas, with lower expression in heart, placenta, lung, and kidney; expression is significantly elevated in colon and lung cancers.
• During herpes simplex virus 1 infection, RIPK3 forms heteromeric amyloid structures with the viral protein ICP6, inhibiting necroptosis and facilitating immune evasion.
• Post-translational modifications include phosphorylation (auto- and trans-phosphorylation with RIPK1), isopeptide bond formation, and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 101–200 of human RIPK3 (NP_006862.2). This region is conserved across human, mouse, and rat, consistent with the observed cross-reactivity.
• For Western blotting, the predicted molecular weight is approximately 57 kDa; however, additional bands may appear due to post-translational modifications (e.g., phosphorylation) or alternative splicing. Validate band specificity using appropriate controls.
• For immunohistochemistry (IHC-P), antigen retrieval methods may be required. Positive control tissues include pancreas or colon/lung tumor samples where RIPK3 is highly expressed.
• In ELISA applications, confirm reactivity with the target protein under your specific assay conditions; consider using recombinant RIPK3 protein or cell lysates as standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research laboratories by providing reliable antibody raw materials for diverse diagnostic and research needs. For RIPK3-related projects, we offer validated monoclonal and polyclonal antibodies, antibody pairs, and ancillary reagents to streamline necroptosis and apoptosis research. Whether you require bulk supply, customized immunoreagents, or assistance in sourcing rare targets, CamelBio serves as your dedicated procurement partner from concept to clinic.