Anti-RIPK2 Polyclonal Antibody for WB, ELISA - O43353

Core Product Specifications and Parameters

Biological Background: RIPK2 Function and Localization

• RIPK2 (Receptor-interacting serine/threonine-protein kinase 2), also known as RICK, RIP2, CARDIAK, and CARD3, is a dual-specificity kinase essential for innate and adaptive immune signal transduction. Related references: PMID:14638696 PMID:17054981 PMID:21123652
• Upon bacterial peptidoglycan sensing, NOD1 and NOD2 recruit RIPK2 through homotypic CARD-CARD interactions, triggering the formation of RIPK2 filaments that serve as signaling platforms. Related references: PMID:17054981 PMID:17562858 PMID:21123652
• Recruited RIPK2 undergoes K63-linked polyubiquitination by XIAP/BIRC2/BIRC3 and M1-linked linear ubiquitination by the LUBAC complex, converting it into a scaffold for downstream effectors. Related references: PMID:22607974 PMID:28545134 PMID:29452636
• These polyubiquitin chains recruit the IKBKG/NEMO adaptor and the TAB2/TAB3-TAK1 complex, leading to IKK phosphorylation and subsequent NF-κB nuclear translocation and transcriptional activation. Related references: PMID:17562858 PMID:22607974 PMID:29452636 PMID:18079694
• The kinase activity of RIPK2 is dispensable for NOD1/NOD2 signaling; its primary role is as a scaffolding protein. Related references: PMID:29452636 PMID:30026309
• RIPK2 localizes to the cytoplasm, cell membrane, and endoplasmic reticulum, and is widely expressed in tissues including heart, brain, placenta, lung, leukocytes, spleen, and kidney.
• In adaptive immunity, RIPK2 participates in T-cell receptor signaling by promoting BCL10 phosphorylation and NF-κB activation. Related references: PMID:14638696
• RIPK2 also mediates Src-dependent tyrosine phosphorylation of ARHGEF2 in NOD2 signaling and regulates RHOA inactivation downstream of NGFR. Related references: PMID:21887730 PMID:26646181

Experimental Guidance and Technical Tips

• The immunogen covers the full-length RIPK2 protein (aa 1–540), which may result in recognition of multiple isoforms or post-translationally modified forms; confirm the band of interest (predicted ~61 kDa) using appropriate controls.
• For Western blotting, validate the optimal antibody dilution for your specific sample and detection system; endogenous RIPK2 expression levels can vary across cell and tissue types.
• Due to the polyclonal nature, expect potential cross-reactivity; verify specificity using RIPK2 knockout or knockdown samples or by peptide blocking.
• In ELISA applications, titrate the antibody against the recombinant immunogen or native target to determine the linear detection range.
• The antibody cross-reacts with mouse and rat RIPK2, but sequence alignment is recommended when extending to other species.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides researchers and diagnostic developers with streamlined access to high-quality antibodies like this anti-RIPK2 polyclonal antibody, which targets a central innate immunity kinase. Beyond single reagents, we support your projects with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—all designed to accelerate your infectious disease and inflammation assay development. Reach out to explore how CamelBio can fulfill your raw-material needs from concept to clinic.