Anti-RIPK1/RIP Rabbit Monoclonal Antibody (KO Validated) for WB, IP, ELISA - Q13546

Core Product Specifications and Parameters

Biological Background: RIPK1 Function and Localization

• RIPK1 (also known as RIP, RIP1, cell death protein RIP) is a serine-threonine kinase encoded by the RIPK1 gene in humans. Alternative splicing produces isoforms with molecular weights of approximately 76 and 71 kDa.
• As a key regulator of tumor necrosis factor (TNF) signaling, RIPK1 controls apoptosis, necroptosis, and inflammatory pathways (PubMed:17703191, PubMed:24144979, PubMed:31827280). Related references: PMID:17703191 PMID:24144979 PMID:31827280
• RIPK1 has dual functions: it acts as a kinase-independent scaffold in TNF-R1 complex I to promote NF-κB activation and cell survival, while its kinase activity is required to drive pro-death complex IIa (apoptosis) and IIb (necroptosis) (PubMed:11101870, PubMed:19524512, PubMed:19524513). Related references: PMID:11101870 PMID:19524512 PMID:19524513
• In necroptosis, RIPK1 acts as an inhibitor of RIPK3-mediated necroptosis by facilitating FADD-dependent recruitment and activation of caspase‑8, which cleaves RIPK1 (PubMed:19524512, PubMed:19524513, PubMed:29440439). Related references: PMID:19524512 PMID:19524513 PMID:29440439
• RIPK1 also contributes to inflammation by promoting transcriptional production of pro-inflammatory cytokines such as interleukin‑6 (IL6) (PubMed:31827280, PubMed:31827281). Related references: PMID:31827280 PMID:31827281
• The kinase phosphorylates downstream targets including RIPK3 (PubMed:19524513) and the MAP3K5 pathway activator DAB2IP at Ser‑728 (PubMed:15310755, PubMed:17389591). Related references: PMID:19524513 PMID:15310755 PMID:17389591
• Subcellular localization is primarily cytoplasmic, with additional association at the cell membrane.
• Post-translational modifications of RIPK1 include phosphorylation, ubiquitination, and glycosylation; mutations in RIPK1 are linked to autoinflammatory diseases.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (amino acids 465–671) of human RIPK1; this region lies downstream of the kinase domain and may recognize both reported isoforms.
• For Western blot (WB), endogenous RIPK1 typically migrates as a doublet around 76 and 71 kDa; due to post-translational modifications and complex formation, additional bands may appear.
• KO validation confirms antibody specificity; including lysates from RIPK1 knockout cells as a negative control is recommended.
• For immunoprecipitation (IP) and ELISA, optimal antibody concentrations should be determined empirically in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and researchers, providing high-quality raw materials and technical support from concept to commercialization. This anti-RIPK1 monoclonal antibody addresses key research areas in cell death, inflammation, and oncology. Beyond individual antibodies, CamelBio’s supply network includes validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare-target raw materials to streamline diagnostic development.